Pyro Gold Chemistry

The Next-Generation Chemistry for Pyrosequencing technology

Pyro Gold is the new chemistry formulation for Pyrosequencing technology. The result of 2 years of research and development, Pyro Gold contains a number of enhancements that improve the performance of Pyrosequencing, especially in the areas of sequencing read length and first-time assay success.

What’s New
By adding Single-Stranded Binding Protein (SSB) to the enzyme mix in optimized quantities, sequencing read lengths have been greatly improved. SSB disrupts secondary structures in the template to be analysed, resulting in significantly longer read lengths. Other changes to the formulation result in increased peak heights, sharper peaks, and reduced background noise. All these factors add up to improve the accuracy and readability of Pyrogram traces.

Pyro Gold increases read length by decreasing drop-off, reducing background,
and disrupting secondary structures. Sequence data from the 16S rRNA gene of
Thiobacillus baregensis comparing the obtained sequence read length when using
the old formulation (upper Pyrogram trace) and the new Pyro Gold formulation (lower
Pyrogram trace). In this case the read length has increased by a factor 3.

Longer Sequencing, Stronger Genotyping
For CpG methylation analysis, Pyro Gold can increase the number of CpG sites that can be quantified in a single assay. Dr. Jörg Tost of Centre National de Génotypage, Evry, Paris, was one of the beta-testers of the new Pyro Gold chemistry. Jörg’s opinion of Pyro Gold:

“I tested a few of our more demanding assays using the new Pyro Gold chemistry, and I am very happy with the quality of the results. I think that Pyro Gold is a further improvement of Pyrosequencing, especially for those who do not have much expertise in the platform. I really like it.” 

 

Dramatically improved sequencing performance with Pyro Gold. This example shows the quantification of methylation in nine CpG sites spanning 70 nucleotides. 


Pyro Gold products are designed for laboratory use only, which means they can be used either for research or by high complexity CLIA-certified labs.

PyroMark Q96 ID and Q96 MD Accessories