IdentiFire Software

Streamlined sequence-based
identification

What is IdentiFire?

IdentiFire software performs sequence-based species identification by comparing and matching Pyrosequencing data to a local database. Users have full control of the content of the database, and can therefore perform searches on sequences either from public sources, e.g. GenBank, as well as sequences validated by their laboratory. IdentiFire offers a major advantage in that the workflow from sequence generation to final identification is fast and intuitive. This makes it an efficient platform for routine and high-throughput microbial identification.

What Problem Does IdentiFire solve?

In order to perform the actual typing, researchers have previously been forced to perform tedious web-based database searches of the generated sequence strings. This is labour intensive, requires a lot of user interpretation of the results due to the limitations in the databases, and the BLAST alignment algorithm is suboptimal for Pyrosequencing data.

IdentiFire supports Identification and Resistance Typing

The software is not limited for use within microbial typing; it can be used for any application where alignments of Pyrosequencing data against libraries are performed. For instance, it can be used for sequence matching to type microbial resistance.

IdentiFire brings you

Convenient data input	Base-called sequence strings can automatically be retrieved from the PSQ software
Pyrosequencing-specific alignment	The alignments against user-generated sequence libraries are performed optimally for Pyrosequencing data, i.e. flexibility for homopolymers, and a larger weight on bases at the beginning of the sequence.
Fast analysis	A full plate of 96 sequences can be set up and analyzed within a minute.
Easily interpreted typing results	A score plus a quality assessment of the hits makes the results easy to read
Practical report formats	Several report formats can be chosen, where annotations can be added by the lab technician to make it suitable as a final report to the clinician.

How IdentiFire Works, Step-by-Step

1. Enter analysis name and notes.
   
   
2. Select the sequence data to be analyzed. Data can be retrieved from the Pyrosequencing database of executed runs, imported from a FASTA file or manually entered.
   
3. Choose the sequence library for the analysis. The sequence library to be used for analysis is either applied to all the samples, or can be individually set for each sample. In other words, several different assays can be analyzed simultaneously.
   
   
4. Run! The analysis takes about 1 minute for 96 samples.

After analysis, a report is automatically generated.

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