Pyrosequencing gives us all the advantages of high quality sequencing, yet in real-time. It gives us both ease of use and quality control that is unavailable by any other genotyping method. Pyrosequencing delivers meaningful data despite the high rate of mutation by microorganisms, making it a very powerful platform for the development of methods to detect antibiotic resistance.

Saheer Gharbia, MD, PhD
Head; Genomic and Proteomic Informatics
Health Protection Agency, London