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Pyrosequencing technology gives an assay for any genetic marker

Flexible assay design
- Minimal limitations in primer placement
Easy primer design
- Only requires a standard biotinylation of one of the PCR primers
- No complicated modifications of oligonucleotides
- Supported by Assay Design Software
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We were told by a major supplier of genotyping technology that an assay for trisomy couldn't be achieved in a quantitative manner. We turned to Pyrosequencing technology, and the result looks beautiful. Pyrosequencing is certainly a facilitator for new and useful genetic analyses in research.
Dr. Neil Lamb, Director, Center for Medical Genomics, Emory University, Atlanta, GA |
An estimated 20-30% of SNPs in large-scale SNP studies are rejected because of failure of the genotyping assay*. Pyrosequencing technology can provide functional assays for most, if not all, difficult SNPs and mutations.
Pyrosequencing technology has an unbeaten record of success for assaying any polymorphism or mutation. Assays can be designed with great flexibility in primer placement. Pyrosequencing assay design is both flexible and simple to do. We provide Assay Design Software to support users.
Pyrosequencing technology therefore gives researchers full freedom to explore genetic markers unhindered by limitations in technology, based on robust assays that are easily translated to standard methods in the research community, and homebrew assays by CLIA-certified labs.
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© QIAGEN 2010. All rights reserved
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