Pyrosequencing assays are mutation-tolerant

Consequence of new mutation:

• With hybridization-based probe, no hybridization occurs in the presence of a mutation
  
  
• With Pyrosequencing technology, new mutations are tolerated and detected
  

Unlike the majority of genotyping technologies, which depend on a probe hybridizing to a specific site of interest, Pyrosequencing assays are tolerant of unexpected mutations.  When PCR primers are placed in conserved regions of DNA, Pyrosequencing assays produce meaningful results, regardless of the mutation.

Pyrosequencing technology is the method of choice for simple and robust assays in studies of antibiotic resistance and the identification of new microbial mutations.