How Can Sequential PCR Tests for Microbial Determination be Improved?

How can sequential PCR tests for Microbial Determination be improved?

Bacterial screen

QPCR is sensitive and highly quantitative, however one probe is required for each species - time-consuming and costly
With Pyrosequencing technology, only 1 sequencing primer is needed for species identification
Combining QPCR for screening with Pyrosequencing technology gives high-speed, high resolution species identification at lower cost

DNA purification

Real-time PCR with SYBR green for Yes/No

If the answer is Yes

Pyrosequencing analysis using PCR product

Species identification (BLAST towards local database)

Real-time PCR and Pyrosequencing technology in combined screening and identification protocols dramatically improve workflow and accuracy of microbial identification, and reduce costs.

Users have demonstrated cost-savings and improved responsiveness when using PCR to screen for microbial load, followed by Pyrosequencing technology for one-step identification.