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| The Strenghts of Pyrosequencing technology |
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 This is how Pyrosequencing technology is unique compared to other genotyping and genetic detection methods:
- It presents an analyzed mutation in the context of the neighbouring genetic sequence – we call this “Built-in Quality Control”. It is a foolproof means of guaranteeing that the assay worked correctly.
- It delivers the "gold standard" of genetic analysis: the sequence itself. Other methods only provide a “Yes/No” signal. Unlike a fluorescent signal, sequence information is intelligible; therefore it is easy to communicate these results in the literature, and easy to transfer meaningful data between research labs.
- With Pyrosequencing technology, there is great flexibility in primer placement. Therefore it is easy to design a Pyrosequencing assay to analyze virtually any genetic marker. So far, Pyrosequencing technology has an unbeaten track record in analysing any SNP.
- Pyrosequencing assays are mutation-tolerant. Unlike hybridisation-based assays, Pyrosequencing analysis generates a correct sequence regardless of the appearance of a new, unexpected mutation. This is very important to microbiological applications: hybridisation-based assays can give false negatives in the presence of a new mutation.
- With Pyrosequencing analysis, you not only obtain sequence information, but the data is also fully quantitative, ideal for measuring the relative amounts of alleles. This property allows the quantification of DNA methylation, heterozygosity, ploidy levels, multi-copy genes, pooled DNA samples, hematopoeitic chimerism, and mixed genotypes in heterogeneous samples (e.g. tumor and normal cells).
- Pyrosequencing technology easily addresses the many and varied applications that are typical for clinical research labs.
Read more about the strengths of Pyrosequencing technology:
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