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| Pyrosequencing technology: As Easy as PCR, and as reliable as Sanger | 
DNA sequence from the P3 region of the rnpB gene of Streptococcus Pyogenes
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...Rapid sequence data that are objective, portable for comparison between laboratories, and reproducible for epidemiological characterization of N. Gonorrheoeae isolates are generated.
Overall, sequence analysis by using high-throughput Pyrosequencing technology is accurate and reproducible and can be almost fully automated. The technology is also less expensive, time-consuming, and labor-intensive, as well as easier to perform, than conventional Sanger sequencing.
(From: Unemo M, Olcen P, Jonasson J, Fredlund H., J. Clin. Microbiol., Jul 2004; 42: 2926 - 2934.)
| | Clinical researchers demand the reliability of true sequence data in order to commit to employing genetic detection methods in a clinical environment. Pyrosequencing technology has been confirmed to be superior to Sanger sequencing in terms of time, cost, quantification, as well as ease-of-use for reading stretches of DNA for discriminatory purposes.
Pyrosequencing technology has thereby enabled the application of sequencing in routine clinical applications. Clinical research in microbiological typing is the primary focus for much of the ongoing clinical research.
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