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Pyrosequencing archive

arrow Technical notes  arrow White Papers
arrow Application notes arrow Presentations
arrow Product information sheets arrow Scientific Publications

arrow Highlights - The Pyrosequencing Newsletter

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Technical notes

2004-12-20 Pyrosequencing for quantitative analysis of CpG methylation
2004-02-28 The principle of Pyrosequencing
 
Application notes
2008-05-10 Rapid and accurate typing of the protozoan parasite Giardia lamblia by Pyrosequencing® analysis
2008-05-29 Microbial Species discrimination and resistance detection by PyroMark ID
2006-01-26 Efficient Influenza A antiviral drug resistance surveillance by Pyrosequencing
2008-10-01 Oncological Applications
2005-01-01 Efficient microbial screening and identification based on real-time PCR and Pyrosequencing
2005-10-01 Pyro Q-CpG: quantitative analysis of methylation in multiple CpG sites by Pyrosequencing
2005-10-01 Microbiological Applications
2004-12-01 Real sequence data for genotyping, as easy to read as A-C-G-T
2004-11-18 Efficient microbial screening and identification based on real-time PCR and Pyrosequencing
     
Product Information
CE-marked under European IVD Directive 98/79/EC
Click here for more information
  2008-03-31 PyroMark™Q24 System -  Europe
Product Information
  2008-06-18 PyroMark Control Oligo and PyroMark Q24 Validation Oligo
  2009-09-10 PyroMark™Q24 RUO tests
  2008-03-31 PyroMark Q24 system  - US and ROW
  2009-11-25 PyroMark Q96/Q24 KRAS v2.0
  2006-03-29 PyroMark MD Instrument
  2006-03-29 PyroMark MD system 
  2006-03-29 Pyro Q-CpG software 
  2005-11-28 Genetic analysis by real-time sequencing of PCR templates
  2005-05-23 PyroMark BRAF
  2005-05-23 PyroMark MLH1
  2005-05-23 PyroMark p16
  2005-03-01 Identifire software
  2005-03-01 PyroMark ID instrument
  PyroMark ID system
  2005-02-01 PyroMark HFE
  2005-02-01 PyroMark APOE
  2005-02-01 PyroMark MTHFR
  2005-02-01 PyroMark PWS/AS
  2004-05-20 Pyrosequencing Assay Design Software
  2004-09-25 Pyrosequencing Vacuum Prep Workstation
White Paper
2007-03-15 CpG Methylation by Pyrosequencing Benchmarks and Application - WP010
2006-01-01 White Paper - Sequencing Microbial Markers for Objective Typing in forensics, Defense and Public Health
2004-04-27 Microbial Identification and Drug Resistance Detection - WP 009
Presentations
2004-05-24 Pyrosequencing for DNA Methylation Analysis
Scientific Publications
Key Publications Oncology
webb page Uhlmann K., Brinckmann A., Toliat M. R., Ritter H., Nürnberg P.
Evaluation of a potential epigenetic biomarker by quantitative methyl-single nucleotide polymorphism analysis. Electrophoresis 23; 4072-9, 2002

Colella S., Shen L., Baggerly K.A., Issa J.-P.J., Krahe R. Sensitive and quantitative universal Pyrosequencing™ methylation analysis of CpG sites. Biotechniques 35; 146-50,2003
Tost J., Dunker J., Glynne Gut Y. Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing™. Biotechniques 35; 152-6, 2003
webb page Dupont JM, Tost J, Jammes H, and Gut IG. De novo quantitative bisulfite sequencing using the Pyrosequencing technology. Anal Biochem, Oct 2004; 333(1): 119-27.
webb page Yang AS, Estecio MR, Doshi K, Kondo Y, Tajara EH, Issa JP. A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements. Nucleic Acids Res. Feb 2004; 32(3): e38.
webb page CpG methylation in clinical studies: utility, methods, and quality assurance
Key Publications Microbiology
webb page Unemo M, Olcen P, Jonasson J, Fredlund H.
Molecular Typing of Neisseria gonorrhoeae Isolates by Pyrosequencing of Highly Polymorphic Segments of the porB Gene. J. Clin. Microbiol., Jul 2004; 42: 2926 - 2934.
webb page Hjalmarsson S, Alderborn A, Fock C, Muldin I, Kling H, Uhlen M, Engstrand L.
Rapid combined characterization of microorganism and host genotypes using a single technology. Helicobacter, Apr 2004; 9(2): 138-45.
Diggle MA and Clarke SC.
Genotypic Characterization of Neisseria meningitidis Using Pyrosequencing. Mol Biotechnol, Oct 2004; 28(2): 139-46.
Mortimer CK, Peters TM, Gharbia SE, Logan JM, Arnold C.
Towards the development of a DNA-sequence based approach to serotyping of Salmonella enterica. BMC Microbiol, Aug 2004; 4(1): 31.
webb page Jordan JA, Butchko AR, Beth Durso M.
Use of Pyrosequencing of 16S rRNA Fragments to Differentiate between Bacteria Responsible for Neonatal Sepsis. J Mol. Diagn., Feb 2005; 7: 105 - 110.

webb page Tärnberg M., Jakobsson T., Jonasson J., Forsum U.
Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions. AMPIS 110: 802-10, 2002

webb page Kolak M, Karpati F, Monstein HJ, Jonasson J.
Molecular typing of the bacterial flora in sputum of cystic fibrosis patients. Int J Med Microbiol. Aug 2003; 293(4): 309-17
webb page Kim A, Tuohy MJ, Gordon SM, Hall GS, Procop GW.
The Use of Molecular Methods to Determine the Cause of Mycobacterial Infections. Pathology Case Reviews, Jul 2003; 8(4): 152-162.
Describes current clinical practice for identification of Mycobacteria, and how molecular methods like Pyrosequencing speed up and improve identification and differentiation
Pyrosequencing™
Sequence Typing at the Speed of Light
Matthew A. Diggle and Stuart C. Clarke*

Genotypic Characterization of Neisseria meningitidis Using Pyrosequencing™
Matthew A. Diggle and Stuart C. Clarke*

 

Highlights - The Pyrosequencing Newsletter
May 2008 Highlights - The Pyrosequencing Newsletter May 2008
December 2007 Highlights December 2007
June 2007 Highlights  June 2007
December 2006 Highlights December 2006
May 2006 Highlights - May 2006
 
 
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