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Application notes and Posters
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This page will be continuously updated as new applications become available.
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| Application Notes |
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 Allele Frequency Quantification using Pyrosequencing™ Systems (TN108) |
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| Sequence analysis (SQA) using the PSQ™96MA System (TN107) |
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| SNP and mutation analysis using Pyrosequencing™ Systems (TN106) |
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| Forensic identification using mitochondrial DNA (AN211) |
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| Typing of bacteria: Bordetella pertussis and parapertussis (AN210) |
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| Easily designed multiplex analysis of SNPs and InDels (TN105) |
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| Reliable determination of haplotypes (TN104) |
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| Identification and virulence characterization of Bacillus anthracis (AN209) |
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| Detection and typing of bacteria: Listeria monocytogenes (AN207) |
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| Estimation of SNP allele frequencies (TN103) |
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| SNP genotyping in codon 72 of the p53 gene (AN204) |
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| SNP analysis of ACE (angiotensin converting enzyme) (AN201) |
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| SNP genotyping of MC1R (melanocortin receptor 1) in pigs (AN202) |
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| Scientific Posters |
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| Using Pyrosequencing™ technology to assess common CFTR mutations |
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| Gene expression analysis using Pyrosequencing™ technology |
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| I23: Molecular Identification of Gram-positive bacteria using Pyrosequencing™ technology |
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| Quantitative analysis of methylation status at CpG islands using Pyrosequencing™ technology |
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Rapid identification and virulence characterization of Bacillus anthracis using
Pyrosequencing™ technology |
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| Molecular haplotype determination using allele-specific PCR and Pyrosequencing™ technology |
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| An assay panel for identification of Cytochrome P450 alleles using Pyrosequencing™ technology |
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| High throughput SNP scoring using Pyrosequencing™ technology |
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| Multiplex Genotyping of SNPs Using Pyrosequencing™ |
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| Performance of PSQ 96 System for SNP scoring |
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| Pyrosequencing: Performance and Application to Factor V Leiden Determination |
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| Manual and Automatic Sample Preparation for Pyrosequencing |
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| Accurate Allele Frequency Estimation of SNPs using Pyrosequencing |
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| Pyrosequencing Technology for Signature Tag Determination |
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| Reliable SNP Assessment and Allele Frequency Determination by Pyrosequencing™ |
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 Allele Frequency Quantification using Pyrosequencing™ Systems (TN108)
- Fast, reliable and proven Pyrosequencing™ technology
- Pooling of genomic DNA samples reduces cost per accurate result and increases efficiency
- Quantitative results
- Data exporting capabilities enhance statistical analysis
To read the complete document download the technical note |
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 Sequence analysis (SQA) using the PSQ™96MA System (TN107)
- Fast, reliable and proven Pyrosequencing™ technology
- Analyze templates with strong secondary structures
- Automatic base-calling and sequence alignment
- Export sequence data for database comparison
To read the complete document download the technical note |
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 SNP and mutation analysis using Pyrosequencing™ Systems (TN106)
- Fast, reliable and proven Pyrosequencing™ technology
- Accurate and unambiguous results
- Multiplexing capabilities reduce cost per accurate result and increase throughput
- Ability to analyze SNPs, insertions/deletions and other
mutations
- Ability to import data reduces input times
To read the complete document download the technical note |
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 Forensic identification using mitochondrial DNA (AN211)
Pyrosequencing™ technology offers a fast and easy to use solution for
forensic identification using mitochondrial DNA. Dependable results
are obtained within days rather than weeks, increasing efficiency
and making Pyrosequencing Systems an attractive alternative to current techniques.
To read the complete document download the application note |
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 Typing of bacteria: Bordetella pertussis and parapertussis (AN210)
Sequence analysis using Pyrosequencing™technology accurately
determines different alleles of the toxin S1 subunit gene in Bordetella pertussis by analysis of three polymorphic positions within 13 bases.
By analysis of a fourth variable position in the same reaction, B. pertussis
and B. parapertussis are clearly distinguished.
To read the complete document download the application note |
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 Easily designed multiplex analysis of SNPs and InDels (TN105)
Pyrosequencing™ technology offers an easily designed solution for multiplex analysis of polymorphisms. The obtained increase in cost-effectiveness and efficiency can be further enhanced by multiplex PCR amplification.
To read the complete document download the technical note |
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 Reliable determination of haplotypes
(TN104)
Pyrosequencing™ technology offers a reliable solution for the determination of several SNPs over a large distance on a single chromosome: haplotyping. Allele-specific PCR is used to amplify the allele of interest. To diminish the risk of amplifying the wrong allele, a mismatch is introduced next to the 3' end of the allele-specific PCR primer.
To read the complete document download the technical note |
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 Identification and virulence characterization of Bacillus anthracis (AN209)
Sequence analysis using PCR and Pyrosequencing™ technology offers a rapid and simple method to reliably identify and determine the virulence status of the anthrax-causing bacterium Bacillus anthracis. Faster than alternative techniques, accurate results are obtained within hours.
To read the complete document download the application note |
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 Detection and typing of baceria: Listeria monocytogenes (AN207)
Pyrosequencing™ technology provides a rapid, on-site solution for detection and typing of the pathogen Listeria monocytogenes by sequence analysis of a short region of the inlB gene.
To read the complete document download the application note |
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 Estimation of SNP allele frequencies (TN103)
Pyrosequencing™ offers a method for the estimation of SNP allele frequencies inpooled sample populations.
To read the complete document download the technical note |
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 SNP genotyping in codon 72 of the p53 gene (AN204)
Pyrosequencing™ provided an easily interpreted, accurate and robust assay for genotyping of the SNP in codon 72 of the p53 tumour suppressor gene. The SNP is located within a GC-rich region of the gene and samples were analyzed from paraffin wax tissue sections.
To read the complete document download the application note |
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 SNP analysis of ACE (angiotensin converting enzyme) (AN201)
Known polymorphisms in the RAAS pathway were investigated using Pyrosequencing technology. Homozygotes and heterozygotes were clearly identified in both ACE exon 15 and exon 8. Results of this analysis, agreed with other methods of genotyping
To read the complete document download the application note |
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 SNP genotyping of MC1R (melanocortin receptor 1) in pigs (AN202)
Pyrosequencing™ technology is able to assign differences in SNP genotypes clearly and accurately. In this study Pyrosequencing was used for the assignment of genotypes for 8 polymorphic positions in 15 pigs from 8 different breeds and crosses.
To read the complete document download the application note |
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