Pyrosequencing Technology

Pyrosequencing technology: fast, qualitative sequencing data simultaneously with quantitative allele frequency information

Pyrosequencing technology has an unprecedented set of properties that makes it uniquely suited and highly powerful for certain applications. In this section, we present a concise description of the technological aspects of Pyrosequencing technology that are unique compared to other genotyping and genetic detection methods.

To see what this means in specific scientific applications, look at the  Applications section.

 Strengths of Pyrosequencing technology 
The unique aspects of the technology compared to other genotyping and genetic detection methods.

 Principle of Pyrosequencing technology 
A description of the Pyrosequencing technology reaction cascade, based on sequencing by synthesis, from the nucleotide incorporation event to the detection of light.

 Synergies between PCR and Pyrosequencing Ananlysis

 DNA Sequencing
Pyrosequencing technology is as easy and as fast as running a PCR reaction, and it produces real sequence information. 
 
 Genotyping and SNP Analysis
Pyrosequencing technology gives fast genotyping results with built-in quality control.

 Allele Quantification
Pyrosequencing technology is the only technology able to provide fully quantitative allele data. This makes it an ideal technology for quantifying gene copies (e.g. multisite variation (MSV), or SNP duplicons), DNA methylation and ploidy levels, amongst other applications.

 Whole Genome Sequencing
Pyrosequencng technology is the chosen detection method behind the Whole Genome Sequencing technology from 454 Life Sciences.