Part No: IST1069AIssued year: 2012File size: 0.11mbFile type: pdf
This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites
ISOLUTE, unknown screening,
Part No: IST1044AIssued year: 2011File size: 0.11mbFile type: pdf
The method addresses the extraction of all three classes of drugs of abuse from urine using non-polar and cation exchange retention mechanisms on the ISOLUTE HCX mixed mode column. This protocol was optimized to eliminate the use of dichloromethane in the elution of the acidic / neutral drug fraction.
Part No: P085Issued year: 2014File size: 0.46mbFile type: pdf
This poster demonstrates a novel, sensitive, cost effective and rugged method for the analysis of acrylamide in coffee and potato chips (crisps) using supported liquid extraction prior to LC-MS/MS
analysis. It demonstrates extraction from coffee down
to a concentration of 1 ng/mL and in potato chips to a level
of 10 ng/g (ppb).
Part No: AN796Issued year: 2013File size: 0.62mbFile type: pdf
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection.
The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb in ground coffee (solid) or 125 ppb in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.
Part No: IST1076AIssued year: 2011File size: 0.12mbFile type: pdf
This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS.
ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE
Part No: AN807Issued year: 2013File size: 1.05mbFile type: pdf
This application note describes an SPE protocol appropriate for LC-MS/MS analysis of aflatoxin M1 found in infant formula.
The method described in this application note achieves high recoveries of aflatoxin M1 from infant formula with %RSDs and LOQs that all meet the requirements set in European regulations for its measurement.
Part No: AN785Issued year: 2014File size: 1.28mbFile type: pdf
This application note describes a solid phase extraction(SPE) protocol for the extraction of a range of mycotoxins from dried chili (pimiento) using ISOLUTE® Myco with LC-MS/MS analysis.
The method achieves high recoveries of aflatoxins and ochratoxin from dried chili (pimiento) with %RSDs and LOQs that meet the requirements set in European Union regulations for measurement of these analytes.
Part No: AN865Issued year: 2016File size: 2.02mbFile type: pdf
This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of amphetamine style compounds from oral fluid matrix collected using the NeoSalTM device, prior to GC/MS analysis.
Part No: AN855Issued year: 2015File size: 1mbFile type: pdf
This application note describes the extraction of a range of amphetamine-type compounds from whole blood, prior to GC/MS analysis. A protocol that allows the simultaneous extraction of various other drugs of abuse classes: barbiturates, benzodiazepines, cocaine and opiates, is also evaluated.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).
Part No: AN827Issued year: 2014File size: 1.73mbFile type: pdf
This application note describes the extraction of a range of amphetamines and metabolites from urine using supported liquid extraction and subsequent analysis by GC/MS. Prior to extraction, a simple oxidation step is performed to eliminate sympathomimetic compounds such as ephedrine and pseudoephedrine so they do not interfere with quantitation of methamphetamine.
Part No: AN742Issued year: 2011File size: 0.21mbFile type: pdf
This method describes the use of ISOLUTE SLE+ in 96 well plate format for the extraction of a range of amphetamines. This procedure is effective with sample volumes of 100 μL for analyte recoveries > 90%. For larger volume (500 μL) samples, see AN746.
amphetamines, AN742, ISOLUTE, SLE+, DOA, DRUGS OF ABUSE, FORENSIC
Part No: AN708Issued year: 2011File size: 0.09mbFile type: pdf
The following method(s) have been developed for the class-selective extraction of Amphetamine, Methamphetamine, Phentermine, MDA, MDMA, MDEA from human urine. The method is highly reproducible and offers an average recovery greater than 80%.
MIP, AFFINILUTE, SupelMIP, BIOTAGE, Amphetamines,
Part No: AN746Issued year: 2011File size: 0.21mbFile type: pdf
This method describes the use of ISOLUTE SLE+ in column format for the extraction of a range of amphetamines. This method is effective for sample volumes of 500μL with analyte recoveries > 95%.
Amphetamines, ISOLUTE, DOA, DRUGS OF ABUSE, SLE+, DRUG SCREEN
Part No: AN731Issued year: 2010File size: 0.09mbFile type: pdf
This procedure is recommended for the extraction of extensive panel of significant therapeutic and illicit drugs of abuse from whole blood and urine using mixed-mode cation exchange SPE columns. ISOLUTE HCX is a silica based mixed-mode sorbent that combines non-polar and strong cation exchange retention mechanisms for extraction of basic drugs from biological fluids.
Ion exchange, Drugs of Abuse, DOA, Clinical,
Part No: AN843Issued year: 2015File size: 0.86mbFile type: pdf
Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses.
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis.
The method achieves high reproducible analyte recoveries from both gelding and filly urine. Protocols for 48-well, 96-well plate and column formats are described.
Part No: P074Issued year: 2014File size: 0.52mbFile type: pdf
Antiepileptic drugs are prescribed to suppress seizures in epilepsy patients. A variety of different types of AEDs have been synthesized to pharmacologically address different types of epilepsy. The ability to therapeutically monitor these drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of thedrug. A fast and clean extraction method is needed that works in a variety of biological
matrices and affords a high throughput workflow. In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96 well plate format to facilitate a high throughput workflow model.
Part No: P082Issued year: 2014File size: 0.63mbFile type: pdf
In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract antiepileptic drugs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96-well plate format to facilitate a high throughput workflow model.