This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

96-well plate Application Notes B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Serum Vitamin B3 Vitamins

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE® ENV+ solid phase extraction columns.

Application Notes Biomarker Clinical Column English LC-MS/MS Mono(2-ethyl-5-carboxypentyl) phthalate (MECPP) Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono(2-ethylhexyl) phthalate (MEHP) Monobenzyl phthalate (MBzP) Monobutyl phthalate (MBP) Monoethyl phthalate (MEP) Monohexyl phthalate (MHxP) Monoisononyl phthalate (MiNP) Monomethyl phthalate (MMP) Phthalate Metabolites Phthalates Urine

This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.

Alcohol metabolite Application Notes Clinical Column Ethyl Sulfate (EtS) Ethyl glucuronide (EtG) Forensic LC-MS/MS Urine

This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetyl codeine 96-well plate Acidic Alprazolam Amphetamine Application Notes Barbiturates Basic Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Clinical Clonazepam Codeine Dextromethorphan Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydrolysed urine Hydromorphone LC-MS/MS MDEA Naltrexone Neutral Nitrazepam Norbuprenorphine Norcodeine Norfentanyl Normeperidine Opiates Oxazepam Oxycodone Oxymorphone Pain Management Pentazocine Pentobarbital Phenobarbital Propoxyphene Secobarbital THC metabolites Temazepam Urine Δ9-tetrahydrocannabinol (THC)

La cromatografía flash en fase normal y fase reversa facilita el aislamiento de compuestos polares presentes en extractos de productos naturales. La combinación de avanzadas funciones de detección tales como barrido espectral, y evaporativo de luz dispersa (ELSD) incrementa la efectividad de la cromatografía flash en procesos de purificación de productos naturales.

Application Notes Spanish

This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates. EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.

6-acetyl codeine 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amitriptyline Amphetamine Amphetamines Analgesics Application Notes Aripiprazole Atomoxetine Barbiturates Benzodiazepines Benzoylecgonine Buprenorphine Bupropion Buspirone Butalbital Carbamate hypnotic Carbamate muscle relaxant Carbamates Carbazepine Carisoprodol Chlordiazepoxide Chlorpheniramine Chlorpromazine Clinical Clomipramine Clonazepam Clonidine Clozapine Cocaethylene Cocaine Cocaines Codeine Cyclobenzaprine Desalkylflurazepam Designer stimulants Dextromethorphan Diazepam Dihydrocodeine (DHC) Drugs of Abuse Duloxetine EDDP English Ethyl Sulfate (EtS) Extrahera Fentanyl Fluoxetine Forensic Gabapentin Haloperidol Hydrocodone Hydrolysed urine Hydromorphone Hydroxyalprazolam Hydroxybupropion Hydroxymidazolam Hydroxytriazolam Imipramine Ketamine LC-MS/MS Lamotrigine Levetiracetam Lidocaine Lorazepam MDA MDEA MDMA Meperidine Meprobamate Methadone Methamphetamine Methaqualone Methcathinone Midazolam Morphine N-des-tapentadol N-desmethylclomipramine N-desmethyltapentadol Naloxone Norbuprenorphine Nordiazepam Norfentanyl Norhydrocodone Norketamine Normeperidine Noroxymorphone Norpropoxyphene Nortryptiline Opiates Opioid Opioid agonist Oxazepam Oxcarbazepine Oxycodone Oxymorphone Pain Management Pentobarbital Pharmaceuticals Phencyclidine (PCP) Phenobarbital Pregabalin Quetiapine Ritalinic acid Secobarbital Stimulants THC-COOH Tapentadol Temazepam Topiramate Tramadol Triazolam Trimipramine Urine Venlafaxine Zolpidem Zolpidem-phenyl-4-COOH mCPP z-drugs

This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.

2,4,5-T 2,4-D 2,4-DB Acid herbicides Application Notes Asulam Benazolin Bentazone Bromoxynil Dicamba Dichlorprop Drinking water English Environmental Ioxynil MCPA MCPB Mecoprop On-line SPE On-line SPE-LC-MS/MS Pentachlorophenol Quinmerac Surface water Triclopyr Water

Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.

Application Notes Column Drinking water English Environmental LC-MS/MS Polyfluorinated acids

This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.

Application Notes Atenolol Atorvastatin Azithomycin Carbamazepine Ciprofloxacin Clarithromycin Diclofenac English Environmental Erythromycin Fluoxetine On-line SPE On-line SPE-LC-MS/MS Pharmaceuticals Prednisolone Ranitidine Sertraline Tolytriazole Water

The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (Immunalysis) oral fluid collection devices. This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 11-nor-9-carboxy- Δ9-tetrahydrocannabivarin (THC-V-COOH) 11-nor-9-carboxy- Δ9tetrahydrocannabinol glucuronide (THC-COOH-g 11-nor-9-carboxy-Δ9-THC glucuronide Application Notes Cannabidiol Cannabigerol Column Drugs of Abuse English Forensic LC-MS/MS Oral fluid Saliva THC THC metabolites THC-COOH Δ9-tetrahydrocannabinol (THC) Δ9-tetrahydrocannabivarin (THCV)

ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction. Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,

Aliphatic hydrocarbos Application Notes Column English Environmental Polyaromatic hydrocarbons Soil

Biotage® Endeavor™ catalyst screening system was used to investigate the asymmetric hydrogenation of 4-aryl coumarins to 4-aryl-3,4-dihydrocoumarin, an important intermediate in the synthesis of endothelin receptor antagonists.

Application Notes

This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual (Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.

96-well plate Application Notes English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Synthetic Cannabinoids (SPICE) TECAN UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) UR144-(5-chloropentyl) Urine XLR-11

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(ghi)perylene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

Cyclosporine is an immunosuppressant used in organ transplants to overcome rejection. Following the transplant of a heart, liver, kidney or lung, a patient must be regularly tested for the rest of their lives. Blood testing intervals vary from 1 month to 6 months and this rapid reference method is ideal for batch tests with the advantage of no metabolite interfer-ence which is always found in immunological methods.

Application Notes Liver

The removal of interferences and sample concentration are essential steps in trace level detection of pesticides, sheep dips, herbicides and PCBs in river water, wastewaters and trade effluents. The following methods were developed by the Environment Agency National Laboratory Service facility at Nottingham using the Biotage RapidTrace for automated solid phase extraction and the TurboVap II for evaporation. The procedures are UKAS accredited and can reach detection limits of 0.001 ug/Litre for some compounds.

Application Notes Environmental Environmental contaminants Organophosphates RapidTrace Water

EPA methods 8260 & 8270 cover the analysis of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in ground & surface waters. This also now includes 1,4-dioxane and PCBs. This application note will focus on the automated SPE of the sample rather than the detection which usually involves GCMS or LCMS. NB The columns and reagents used for soils and sediment analysis differ to ground water extraction.

Application Notes Environmental Environmental contaminants RapidTrace Soil

The extraction and segregation of Organic compounds from core samples taken from marine sediments is used to predict climate conditions, reconstruct sea temperatures and identify the type of vegetation from the C3 ( Temperate cli-mate) and C4 ( Tropical Climate) isotopic mass spectroscopy ratio analysis.

Application Notes

With the widespread clinical use of antidepressant drugs comes the need to accurately measure therapeutic lev-els to determine proper dosage, as well as check for toxic levels and patient compliance. More and more laboratories are shifting their therapeutic drug monitor-ing from liquid extraction to solid phase extraction. A large clinical laboratory has automated the process of extracting these drugs from serum using the RapidTrace SPE Workstation.

Application Notes

When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.

Application Notes Benzoylecgonine Column Drugs of Abuse Forensic GC-MS RapidTrace Urine