Part No: PN43Issued year: 2011File size: 0.27mbFile type: pdf
Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal.
Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011
Part No: P025Issued year: 2008File size: 0.35mbFile type: pdf
Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits.
Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic
drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined
in terms of protein and phospholipid removal.
Part No: AN707Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of β-blockers from both biological matrices and water. The methods are highly reproducible and offer β-blocker recoveries of > 80%. The method minimizes matrix effects and offers limits of detection of less than 5 ppt in water and less than 10 ppt for plasma and urine.
AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP, BIOTAGE,
Part No: AN719Issued year: 2011File size: 0.09mbFile type: pdf
This procedure is recommended for the extraction of cocaine and its principle metabolites (norcocaine, benzoylecgonine, ecgonine methyl ester, anhydroecgonine methyl ester and cocaethylene) from whole blood, plasma and urine using polymeric mixed-mode cation exchange SPE columns.
Part No: AN713Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of the tobacco specific nitrosamine, NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) from aqueous sample matrices such as biological fluids (e.g., urine). The method is highly reproducible and offers NNAL recoveries in the range of 90% and a limit of detection below 5 ppt.
AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP
Part No: AN733Issued year: 2011File size: 0.21mbFile type: pdf
This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible.
food scare, Melamine, China, kidney failure, renal failure, protein, Biotage, EVOLUTE,
Part No: AN743Issued year: 2011File size: 0.16mbFile type: pdf
This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%.
SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA
Part No: AN706Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed for the selective extraction of beta-agonists from biological matrices. Methods have been developed for both biological tissues (e.g. bovine muscle) and fluids (e.g. urine). The methods are highly reproducible and offer β-agonist recoveries in the range of 35-90%.
MIP, Molecularly Imprinted Polymers, BIOTAGE, AFFINILUTE, SupelMIP,
Part No: AN747Issued year: 2011File size: 0.18mbFile type: pdf
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis.
SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
Part No: AN727Issued year: 2011File size: 0.11mbFile type: pdf
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: IST1072SIssued year: 2006File size: 0.03mbFile type: pdf
This method describes the extraction of quaternary amines, strongly basic amines and amines that are unstable at high pH levels, from biological fluids. The mechanism is mixedmode
hydrophobic and weak cation exchange, allowing acidic elution conditions.