Part No: AN003-HORIssued year: 2009File size: 0.86mbFile type: pdf
This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly
Part No: AN814Issued year: 2014File size: 1.19mbFile type: pdf
This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.
Part No: AN818.v1Issued year: 2014File size: 1.54mbFile type: pdf
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
Part No: AN067Issued year: 2012File size: 1.01mbFile type: pdf
La cromatografía flash en fase normal y fase reversa facilita el aislamiento de compuestos
polares presentes en extractos de productos naturales. La combinación de avanzadas funciones
de detección tales como barrido espectral, y evaporativo de luz dispersa (ELSD) incrementa la
efectividad de la cromatografía flash en procesos de purificación de productos naturales.
Part No: AN886Issued year: 2017File size: 1mbFile type: pdf
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
Part No: AN863Issued year: 2016File size: 1.62mbFile type: pdf
This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.
Part No: SSI-LCMS-096Issued year: 2018File size: 0.87mbFile type: pdf
Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.
Part No: AN862Issued year: 2016File size: 1.48mbFile type: pdf
This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.
Part No: AN815Issued year: 2014File size: 1.52mbFile type: pdf
The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (Immunalysis) oral fluid collection devices.
This application note describes effective and efficient
ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.
Part No: AN703Issued year: 2011File size: 0.04mbFile type: pdf
ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction.
Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,
Part No: AN100Issued year: 2015File size: 0.16mbFile type: pdf
Biotage® Endeavor™ catalyst screening system was used to investigate the asymmetric hydrogenation of 4-aryl coumarins to 4-aryl-3,4-dihydrocoumarin, an important intermediate in the synthesis of endothelin receptor antagonists.
Part No: AN893Issued year: 2018File size: 0.91mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns.
The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: AN892Issued year: 2018File size: 0.86mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns.
The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: AN793Issued year: 2013File size: 2.04mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are
typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual
(Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.
Part No: AN877Issued year: 2017File size: 2.61mbFile type: pdf
This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Part No: AN909.V.1Issued year: 2014File size: 1.37mbFile type: pdf
Cyclosporine is an immunosuppressant used in organ transplants to overcome rejection. Following the transplant of a heart, liver, kidney or lung, a patient must be regularly tested for the rest of their lives. Blood testing intervals vary from 1 month to 6 months and this rapid reference method is ideal for batch tests with the advantage of no metabolite interfer-ence which is always found in immunological methods.
Part No: AN908.V.1Issued year: 2014File size: 1.33mbFile type: pdf
The removal of interferences and sample concentration are
essential steps in trace level detection of pesticides, sheep
dips, herbicides and PCBs in river water, wastewaters and
trade effluents. The following methods were developed by
the Environment Agency National Laboratory Service facility
at Nottingham using the Biotage RapidTrace for automated
solid phase extraction and the TurboVap II for evaporation.
The procedures are UKAS accredited and can reach detection
limits of 0.001 ug/Litre for some compounds.
Part No: AN904.V1Issued year: 2014File size: 1.33mbFile type: pdf
EPA methods 8260 & 8270 cover the analysis of polychlorinated
dibenzo-p-dioxins and polychlorinated dibenzofurans
in ground & surface waters. This also now includes
1,4-dioxane and PCBs. This application note will focus on
the automated SPE of the sample rather than the detection
which usually involves GCMS or LCMS. NB The columns
and reagents used for soils and sediment analysis differ to
ground water extraction.