Part No: AN003-HORIssued year: 2009File size: 0.86mbFile type: pdf
This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly
Part No: P044Issued year: 2012File size: 0.89mbFile type: pdf
The objective was to develop a GC-MS assay for the determination of free benzodiazepines using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods.
ISOLUTE, SLE, SLE+, Supported Liquid Extraction, Benzodiazepines, Forensic, Drugs, DOA, Drugs of Abuse, SOFT,
Part No: AN893Issued year: 2018File size: 0.91mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns.
The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: AN892Issued year: 2018File size: 0.86mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns.
The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: AN877Issued year: 2017File size: 2.61mbFile type: pdf
This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Part No: AN110-HORIssued year: 2016File size: 2.15mbFile type: pdf
This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.
Part No: AN002-HORIssued year: File size: 0.94mbFile type: pdf
This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.
Part No: RP-DS-04Issued year: 2010File size: 0.19mbFile type: pdf
When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: AN009-HORIssued year: 2015File size: 1.33mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN038-HORIssued year: 2009File size: 2.13mbFile type: pdf
This application note demontrates that the Biotage® fully automated extraction, drying and concentration systems used with the Atlantic™ HLB disk are capable of extracting PAH compounds from sea water.
Typical extraction times using the Biotage® Horizon 4790 range from 20 to 25 minutes while drying and concentration on the DryVap® with the DryDisk® took approximately 35 minutes.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: AN004-HORIssued year: 2009File size: 0.72mbFile type: pdf
This evaluation study was conducted to determine the feasibility of the Biotage SPE-DEXÒ 4790 Automated Extraction System for the extraction of herbicides and pesticides. The preliminary results demonstrate the capability of this method for the automated SPE extraction of organic compounds.
Part No: AN101-HORIssued year: 2016File size: 1.07mbFile type: pdf
This application note highlights a SPE disk which was specifically designed to increase the recoveries of traditionally difficult polar compounds as well as help to increase the diversity of the traditional 525.2 list to include those compounds which are of concern in today’s society.
Part No: P171Issued year: 2017File size: 0.69mbFile type: pdf
This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids.
SOFT 2017, Boca Raton
Part No: AN028-HORIssued year: 2010File size: 0.8mbFile type: pdf
Method 1668A is used for determining the level of chlorinated biphenyl congeners by high resolution gas chromatography with high resolution mass spectrometry (HRGC/HRMS). For aqueous samples (samples containing less than 1% solids) – Stable isotopically labelled analogs of the toxic PCB’s are spiked into a 1L sample, and the sample is extracted using the Biotage® Horizon 4790 Automated Extraction System along with the Atlantic™ C18 solid phase extraction (SPE) disk.
Samples are then dried and concentrated using the DryVap® Automated Drying and Concentrating System with DryDisk® technology.