This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.

Acenaphthylene Alachlor Aldrin Alpha-Chlordane Anthracene Application Notes Atrazine Benzo (a) Anthracene Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Bromacil Butylbenzylphthalate Di-(2-ethylhexyl) Phthalate Di-n-butylphthalate Dibenz (a,h) Anthracene Diethyl phthalate Dimethylphosphate (DMP) Disk Drinking water Endrin English Environmental GC-MS Gamma-BHC Gamma-Chlordane Heptachlor Hexachlorobenzene Hexachlorocyclopentadiene Indeno (1,2,3-cd) Pyrene Methoxychlor Metolachor Organic Compounds Pentachlorophenol Phenanthrene Prometon Propachlor Pyrene Simazine Trans-nonachlor Trifluralin

This application note uses the Biotage® Horizon 4790 automated extraction system with a DVB SPE disk to extract an aqueous sample for PAH compounds analysis. The extract is dried using the Horizon Technology DryDisk®.

1-methylnaphthalene 2-methylnaphthalene 3-methylcholanthrene Acenaphthene Acenaphthylene Acenapthalene Anthracene Application Notes Benzo (a) Anthracene Benzo (a) Pyrene Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene English Environmental Fluoranthene GC-MS Napthalene PAHs Perylene-d12 Phenanthrene Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Water

This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.

2,4,6-trichlorophenol 2,4-D 2,4-dichlorophenol 2,4-dimethylphenol 2,4-dinitrophenol 2,6-dichlorophenol 2,6-dinitro-2-methylphenol 2-methylphenol 2-nitrophenol 3-methylphenol 4-chloro-3-methylphenol 4-methylphenol 4-nitrophenol Acenapthalene Acid herbicides Ametrine Anthracene Application Notes Arachlor Atrazine Base-neutral herbicides Bentazone Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Desethyl atrazine Desisopropyl atrazine Dibenzo(ah)anthracene Dibenzylbutyl phthalate Dibutyl phthalate Diethyl phthalate Dimethyl phthalate Dioctyl phthalate English Environmental Environmental contaminants Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Indeno(123-cd)pyrene LC-MS/MS MCPA MCPB Metribuzin Molinate Organochlorine pesticides (OCP) Organophosphate Pesticide Organophosphates PAHs Persistant organic pollutants (POP) Pesticides Phenanthrene Phenols Phthalates Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Product Notes Pyrene Water

US EPA method 550.1 is a method used by regulatory agencies to determine PAH’s in drinking water by liquid-solid extraction (LSE) and high performance liquid chromatography (HPLC).1 The method outlines the steps needed to perform extractions of PAHs from drinking water sources and finished drinking water; as well as the quality control (QC) program requirements to ensure the method is operated under control. Use of Biotage automated sample preparation products allows users to achieve the precise and consistent data needed to comply with EPA method 550.1 QC specifications as stated in method, section 10.3.2., for Initial Demonstration of Capability (IDC); while at the same time, streamlining laboratory practices to increase lab productivity.

4790 Acenaphthene Acenaphthylene Acenapthalene Anthracene Application Notes Benzo (a) Anthracene Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Chrysene Dibenz (a,h) Anthracene Disk Drinking water EPA 550.1 Analytes English Environmental Fluoranthene Fluorene HPLC Indeno (1,2,3-cd) Pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Pyrene