When it comes to polar organic compound purification, many chemists turn to normal-phase flash chromatography often utilizing dichloromethane and methanol as the eluting solvents. While this can work, it often can be challenging to optimize due to methanol’s high polarity and protic chemistry.
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs. Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Ease of use is what stands out as the top feature of Isolera™ flash chromatography system for Professor Anna Bernardi, head of the synthetic organic chemistry research group at the University of Milan. Her current research goal: developing sugar-like molecules, called glycomimetics, for healthier life.
User Case: Kissei Pharmaceutical Co., Ltd. One of Japan’s innovative pharmaceutical companies uses Isolera™ flash purification systems and Biotage® Initiator+ microwave synthesizers in the development of new prescription drugs. Modern lab instruments contribute to efficient use of time and resources at Kissei Pharmaceuticals.
Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.
Isolera Spektra provides definitive fraction purity assessment in both 2-D and 3-D prior to any post flash analysis. With this information chemists quickly know if a fraction contains a pure product suitable for further mass and structure confirmation or whether the impure fractions must be re-purified. This knowledge improves synthesis throughput and avoids any embarrassment of submitting impure fractions for analytical evaluation.
Elution of polar molecules in flash chromatography sometimes requires solvent additives, making purification more cumbersome. The Isolera™ family of flash purification instruments and Biotage® SNAP KP-NH cartridges significantly reduce the extra work involved.
The term “Green Chemistry” has become a major part of the science community’s lexicon. In this application note we will look at two areas for flash chromatography: 1. Replacing chlorinated solvents with those considered more environmentally friendly. 2. Reducing solvent use and waste generation with more thoughtfully applied chromatography principles.
As reversed-phase flash chromatography gains traction in medicinal chemistry labs the need to monitor its cost and safety are becoming more important. Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile – each have advantages and disadvantages.
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration. ACS 2016
Pyrazines are a class of organic molecules often used to provide flavor to foods. They are typically synthesized but some are found in fruits and vegetables, e.g. grapes, bell peppers, peas, asparagus, beetroot, tobacco, and roasted foods. Pyrazine’s heterocyclic chemistry can yield some challenges to their purification due to the various separation kinetics between the compound and silica. Biotage SNAP Ultra.
User report: Flash instruments. Chugai Pharmaceutical uses Biotage flash chromatography products for drug discovery research. When deciding to convert from manual open-column procedures to automated systems, they chose successive generations of Biotage products, ranging from the Flash+® packed column to the Biotage® Horizon, SP1, Isolera™ Spektra, and Isolera™ Dalton automated flash chromatography systems.