Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (300 Å) to provide increased resolution and effective separation of complex peptide mixtures. Depending on the quality of the peptides synthesized, flash chromatography can be used either as the sole method of purification or as a front end clean-up prior to RP-HPLC. SNAP Bio offers close to prep-HPLC performance in a flash format at a fraction of the cost
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. These solutions make your workflow more efficient and improve the quality of peptides you synthesize. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.