Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices.
Data reliability in forensic toxicology is of paramount importance. The possibility of false positives and/or false negatives can have wide ranging consequences and impacts decisions on various parts of the analytical method development process. A poster presented at TIAFT 2016 in Brisbane, Australia, compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis, putting emphasis on the potential for 96-well cross contamination and strategies for its elimination.
- This poster describes effective use of a novel sample preparation system for both column and 96-well plate format and strategies for the elimination of cross contamination.
- Analyte volatility is a key component to the potential for cross contamination.
- Evaporative cross contamination can be affected by gas flow, temperature, nozzle height and solvent properties.
- The Biotage® ACT plate has proved effective for reducing or eliminating evaporative cross contamination as shown by the analyte “hot spot” experiments.
- The Extrahera automated sample preparation platform functions with 24-column and 96-well arrangement for the adaptation of manual extraction methods.
Download the poster below.