Recommended operating procedure for ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates:
If insufficient mixing occurs on sample addition, proteins will not precipitate efficiently in the well. This can lead to turbidity (cloudiness) in extracts or well blockage during processing. For maximum phospholipid removal, do not exceed the vacuum/pressure conditions. For very viscous samples, a slight increase in pressure or vacuum may be required. The use of high vacuum/pressure conditions may lead to breakthrough of matrix components.