An effective and efficient ISOLUTE SLE+ protocol optimized the for 1 mL sample capacity column formats is described in this application note. The simple sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQ from 10 ng/mL.
ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
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Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+