This application note describes the extraction of cocaine and selected metabolites from oral fluid matrix following the collection with the NeoSal™ devices, prior to GC/MS analysis.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation. 

This application note describes an effective and efficient ISOLUTE SLE+ protocol optimized for 1 mL sample volumes. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 76% with RSDs lower than 10% for all analytes.

Analytes

• Anhydroecgonine methyl ester (AEME)
• Ecgonine (EME)
• Norcocaine
• Cocaine
• Cocaethylene
• Benzoylecgonine-D₃ (BZE-D₃)
• Benzoylegonine (BZE)

Download the Application Note below »