The primary goal of the presented method was a rapid screening procedure with high degree of specificity, eliminating cross-reactivity issues, and screening for a wider range of compounds than ELISA. Achieving the goal was particularly challenging because it involved rapid extraction, separation, and detection of multiple classes of compounds.
"As a result, several extraction procedures were evaluated (Liquid-Liquid Extraction, Solid Phase Extraction, Micro- Extraction in Packed Syringes, and SLE+). All of these extraction procedures are viable choices, but the choice of SLE+ was based on reaching the above goals with the best fit into the workflow of the laboratory."
"The simplicity of the three step process (load the sample, wait for 5 minutes, elute) is what makes this procedure very appealing."
"The combination of low sample volume with fast processing and analysis makes this method a suitable replacement for immunoassay screening of the targeted drug classes, while providing far superior specificity and better limits of detection than can routinely be obtained by immunoassay."
Eshwar Jagerdeo, Jason E.Schaff, Rapid screening for drugs of abuse in biological fluids by ultra high performance liquid chromatography/Orbitrap mass spectrometry, Journal of Chromatography B http://dx.doi.org/10.1016/j.jchromb.2016.05.010