The Association for Mass Spectrometry: Applications for the Clinical Lab gather a large following from the analytical chemistry industry to their conferences held around the world. On the week of February 21, an international meeting was held in Palm Springs, California, where Biotage presented four new studies on accelerated analytical workflow, focusing on drug screening and metabolite extraction using ISOLUTE® SLE+ supported liquid extraction protocols.
All posters are available for download below.
The screening for elevated levels of methylmalonic acid (MMA) in serum is commonly used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in humans. MMA is commonly analyzed using LC-MS/MS with or without prior derivatization. This poster summarizes various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. A fast, reliable and robust sample preparation method that could be implemented to extract these drugs from complex biological matrices with good analyte recovery and minimum matrix effects would be ideal for toxicology labs. This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine, oral fluid and whole blood.
The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LCMS-MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS. This poster will demonstrate the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS-MS.
Estrogen, androgen, and glucocorticoid metabolism can be used to assess overall hormonal balance during hormone therapy. Urine is the recommended testing matrix for quantification of primary estrogen levels as well as secondary estrogen metabolites when monitoring overall hormone balance, therapy, and detoxification. Non-invasive collection allows for sampling over a 24-hour period, providing insight into a patient’s circadian rhythm. Since many samples are generated for a single patient in any one day, a fast and robust testing protocol is needed for extraction and analysis during clinical testing. Here, we demonstrate a rapid and reliable sample preparation method using Support Liquid Extraction (SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.