Structural and functional studies of membrane proteins remain a challenge due to difficulties in their handling. The combination of using high concentrations of reagents (0.5M) and fast and precise microwave heating to increase the coupling efficiency using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer, enabled the efficient synthesis of peptide ‘18A’ and simplified the subsequent purification by RP-HPLC despite its relatively low solubility, to afford the desired peptide in 52% yield (468 mg). Read the full application note using the link below.
TurboVap® in German
Biotage Acquires Horizon Technology
Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
Video Demonstration: Multi Racks for TurboVap®
Effective Purification of Peptides using Normal Phase Flash