Certain compounds lacking conjugated double bonds are poorly resolved with UV detection, making chromatographic fractionation challenging with this technique. Examples are sterols and protected carbohydrate derivatives, typically saturated with varying levels of hydroxyl functionality.
Two application notes from Biotage show that an ELSD unit used with a flash chromatography system will dramatically improve detection of these compounds. Because ELSD measure the abundance of particulates passing through the detector, it is more reliable for the detection of organic compounds, especially those with poor chromophores. In a chemical workflow process, this will lead to increased yields and higher purities.
New Findings presented at ASMS 2018
Webinar: A Roadmap to Successful Flash Chromatography
Evaporate Water from Reaction Mixtures in Less Than 10 Minutes
Evaporation of Heat-sensitive Compounds
Quick and Easy Removal of High Boiling Solvents