This paper looks at the impact of different solvents on ionization and explores what happens when samples containing components not optimal for mass detection are used. Questions such as "What if we use a modifier?", "Does a different reaction or sample solvent affect the ionization in Isolera™ Dalton?", " Will I get ion suppression?" are investigated in this extensive study.
Suppression is known to cause problems in ion detection and sensitivity issues are common in many spectroscopic mass ionization techniques. The purpose of this study was to investigate whether ion suppression would affect the way in which Isolera™ Dalton performed, during the process of screening and mass directed flash purification. The results show Isolera Dalton was essentially unaffected by the nature of the solvent used to dissolve or introduce the sample, and also the ionic content of the mixtures.
Using Isolera™ Dalton, all that is required to perform a successful mass directed purification is the ability to recognize a single m/z peak that corresponds to the product of interest. The relative peak height of the chosen m/z is unimportant in triggering fractionation. It was also observed that the act of running flash purification also separates common ion-suppression agents from the compound of interest, further facilitating successful ionization and mass directed flash purification. Thus, Isolera Dalton was able to perform even under the most difficult conditions.