GHB is sometimes described as a ‘date rape’ drug for its correlation with sexual assault cases. It also has alleged widespread use in the dance club scene.
This application note describes the extraction of GHB from urine using supported liquid extraction and subsequent analysis by GC/MS. ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
GC/MS chromatography for GHB in urine (Cal 1) spiked at 2.5 ng/mL. Compared to other more traditional solid phase extraction procedures, this ISOLUTE® SLE+ method results in a baseline with reduced noise and thus offers greater confidence when qualifying GHB.