"Before we get into our extractions, we need to set up our LC/MS to detect our analytes of interest. The LC will provide the chromatography (separation of our analytes) and the MS will support detection. So which one do we evaluate first? Well, if you can’t “see” the analytes, what good is perfecting their chromatography? So, first, let’s get the MS focused, or tuned, for optimum analyte detection."
In this blog post, Applications Chemist Ph.D. Dan Menasco goes into all the details of the mass spec parameters for optimizing a method for sample preparation, and concludes:
"While we could have used the parameters in our poster, its best to tune your analytes on your instrument. Why? Because we need to tune our analytes against the current condition of our instrument, not some else’s."
Perfecting Your Mass Spectrometer Parameters
Sample Preparation Strategies for Urine Panels with 50 Drugs
Biotage Synthesizer Peer-reviewed Literature
How to Identify Compounds in a Mass Spectrum
Release of EVOLUTE® HYDRO SPE Plates with Integral Hydrolysis Capability