A poster (P165) presented at ASMS 2017, Indianapolis, discusses three sample preparation methodologies for 52 drug analytes, and presents evaluations of recovery, matrix effects and process efficiency.
While “dilute and shoot” methods are easy and affordable, they can result in shortened LC column lifetimes and increased MS instrumentation downtime. Matrix effects can also effect sensitivity and overall method performance. Supported Liquid extraction (SLE) and Solid Phase Extraction (SPE) provide cleaner samples for LC-MS/MS and more robust clinical assays.
Successful sample preparation method development requires understanding the retention mechanism of the analytes in the assay. The octanol-water partition coefficient (logP) and acid dissociation constant (pKa) of the compounds of interest are important properties that can guide sample preparation strategy and optimization of method conditions.
New Application Note for Simultaneous Extraction of Aldosterone and Angiotensin I and II from Human Plasma
Get Rid of Peptide Cleavage Cocktail in Three Minutes
The Secret Behind Green Purification
Two New Application Notes for Extraction of 1-nor-9-Carboxy-Δ9-THC from Hydrolyzed Urine
Convert Your TurboVap® LV Into a TurboVap® II