Some chemists think the internal volume of the cartridge without packing material inside is the column volume. While useful in determining scale-up factors, the empty column’s volume is not the CV. The CV of any column or cartridge is the volume inside of a packed cartridge not occupied by the media. This volume includes both the interstitial volume (volume outside of the particles) and the media’s own internal porosity (pore volume). Combined, the two volumes constitute 70% to 80% of the packed cartridge’s volume. Of course this means that the media only occupies 20% to 30% of the space in the cartridge.
Read the full post on the Flash Puricifation Blog, where this concept is fully explained and how it is determined empirically.
TurboVap® in German
Biotage Acquires Horizon Technology
Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
Video Demonstration: Multi Racks for TurboVap®
Effective Purification of Peptides using Normal Phase Flash