The described method allows simultaneous extraction of six metabolites, using a high throughput load-wash-elute/no blow down EVOLUTE® EXPRESS method. The method is automated using Biotage® Extrahera™ sample processor (see full details in the application note). High reproducible recoveries were achieved for all analytes.
EVOLUTE® EXPRESS SPE products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.
See also poster P155, presented at the MSACL meeting in January 2017.