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The Definitive Flash Cartridge Guide

20 November 2014

Which cartridge should I use? How do different silica gels influence my purification? What is the correlation between the Rf value and column volume? What's the difference between isocratic and step gradients? Did you know there are seven ways to load a flash cartridge? 

 

The Definitive Guide to Flash Chromatography gives you all the background on different loading and cartridge options for flash chromatography, and presents the basic theory you need to know for successful flash purification. Conversion from TLC to Column Chromatography

Conversion from TLC to Column Chromatography. Compound 1 has an Rf of 1, and since Cv=1/Rf, the a single column volume of solvent is all that is needed to elute Compound 1. Compound 2 has an Rf of 0.5 and since CV=1/0.5 = 2, Compound 2 will elute in two column volumes. Finally Compound 3 has an Rf of 0.2 resulting in a CV of 5 meaning that it will take 5 column volumes of solvent to elute Compound 3.

Loading a Biotage Samplet flash cartridge

Samplets offer a convenient way to introduce soluble samples dissolved in a volatile solvent. Pour the dissolved material on a Samplet and allow the solvent to evaporate. The Samplet is placed inside of the SNAP cartridge which maximizes the amount of media available for purification and eliminates precipitation risk.

Syringe soading a Biotage Zip® flash cartridge

Using a syringe to load a cartridge is straightforward. Biotage cartridges have Luer inlet fittings for connection to Luer syringe.

Loading a Biotage SNAP Ultra flash cartridge

Sometimes, it is more convenient to adsorb materials for purification onto a solid support such as Biotage HM-N Diatomaceous Earth or silica. For this approach the internal loading directly into a SNAP cartridge is the preferred approach.

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