Drug screening using oral fluid has gained popularity over recent years due to its simple, non-invasive collection means. Screening drugs of abuse can be complicated due to the wide variation of functional groups associated with different analyte classes. Most extraction techniques cannot extract all analytes using a single procedure without using non-optimal extraction protocols, resulting in compromised extract cleanliness. SLE allows for the simultaneous analysis of cross-functional analytes in a single extraction protocol without forfeiting extract cleanliness. This poster demonstrates the extraction of a range of drugs of abuse prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine.