This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, English, Forensic, GC-MS, Hydrolysed urine, THC metabolites, THC-COOH

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.
Tags: 11-deoxycorticosterone, 11-deoxycortisol, 17-OH-Pregnenolone, 17-alpha-hydroxyprogesterone, 18-OH-Corticosterone, 21-deoxycortisol, 96-well plate, Aldosterone, Androstenedione, Application Notes, Clinical, Corticosterone, Cortisol, Cortisone, DHEA (dehydroepiandrosterone, DHEAS, Dehydroepiandrosterone (DHEA), Dihydrotestosterone (DHT), English, Estradiol, Estrogens, Estrone, LC-MS/MS, Pregnenolone, Progesterone, Serum, Steroid Hormones, Steroid metabolite, Testosterone

This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE® SLE+ protocol optimized for the 200 μL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application note achieves high reproducible recoveries (>80%, RSD < 5%) for these analytes in plasma with linearity > 0.995 in the range 2–200 ng/mL.
Tags: 5-hydroxyindoleacetic acid (5-HIAA) , 96-well plate, Acidic catecholamine metabolites, Application Notes, Clinical, English, Homovanillic acid (HVA), LC-MS/MS, Plasma, Vanillylmandelic acid (VMA)

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization.
Tags: Amphetamine, Amphetamines, Application Notes, Bead Ruptor, Clinical, Column, Drugs of Abuse, English, Forensic, GC-MS, Hair, MDA, MDEA, MDMA, Methamphetamine

Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Tags: 96-well plate, Alcohol metabolite, Application Notes, Clinical, English, Extrahera, Forensic, LC-MS/MS, PEth, Whole blood

A wide selection of abstracts from our best application notes in the analytical chemistry business
Tags: Application Notes

This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns. A protocol has been developed that allows the simultaneous extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC. In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Tags: Alprazolam, Amphetamine, Application Notes, Atrazine, Bead Ruptor, Bendiocarb, Biphenthrin, Butabarbital, Chlorothalonil, Clinical, Clonazepam, Cocaine, Cocaines, Column, Diazepam, Drugs of Abuse, English, Estazolam, Forensic, GC-MS, Ketamine, Liver, MDMA, Malathion, Methadone, Methamphetamine, Midazolam, Nitrazepam, Pesticides, Phenobarbital, Propanil, Secobarbital, THC, Triazolam

This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used. High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
Tags: 96-well plate, Application Notes, Clinical, English, Estradiol, Estrogens, Estrone, LC-MS/MS, Serum, Steroid Hormones

This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetyl codeine, 96-well plate, Acidic, Alprazolam, Amphetamine, Application Notes, Barbiturates, Basic, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Clinical, Clonazepam, Codeine, Dextromethorphan, Diazepam, Drugs of Abuse, EDDP, English, Fentanyl, Flunitrazepam, Forensic, Hydrocodone, Hydrolysed urine, Hydromorphone, LC-MS/MS, MDEA, Naltrexone, Neutral, Nitrazepam, Norbuprenorphine, Norcodeine, Norfentanyl, Normeperidine, Opiates, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Pentazocine, Pentobarbital, Phenobarbital, Propoxyphene, Secobarbital, THC metabolites, Temazepam, Urine, Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction of buprenorphine and norbuprenorphine from whole blood using ISOLUTE® SLE+ Supported Liquid Extraction prior to LC-MS/MS analysis.
Tags: 96-well plate, Application Notes, Buprenorphine, Clinical, English, Forensic, LC-MS/MS, Norbuprenorphine, Pain Management, Whole blood

This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 1, 25 Dihydroxyvitamin D, 96-well plate, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin D

This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Tags: Anhydroecgonine Methyl Ester AEME, Anhydroecgonine methyl ester, Application Notes, Benzoylecgonine, Clinical, Cocaethylene, Cocaine, Cocaines, Column, Drugs of Abuse, Ecgonine Methyl Ester EME, English, Forensic, GC-MS, Whole blood

This application note describes the extraction of a range of amphetamine-type compounds from whole blood, prior to GC/MS analysis. A protocol that allows the simultaneous extraction of various other drugs of abuse classes: barbiturates, benzodiazepines, cocaine and opiates, is also evaluated. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).
Tags: 2C-B, 5-APB, 6-APB, Amphetamine, Amphetamines, Application Notes, BZP, Butylone, Clinical, Column, English, Ethylone, Forensic, GC-MS, MDA, MDEA, MDMA, MDPV, Mephedrone, Methamphetamine, Methcathinone, Methedrone, Methylone, Naphyrone, TFMPP, Whole blood, mCPP, pMMA

This application note describes the extraction of benzodiazepine compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, cocaine and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).
Tags: 2-OH-Et-Flurazepam, 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Application Notes, Benzodiazepines, Bromazepam, Clinical, Clonazepam, Column, Diazepam, English, Estazolam, Flunitrazepam, Flurazepam, Forensic, GC-MS, Lorazepam, Midazolam, Nitrazepam, Nordiazepam, Oxazepam, Temazepam, Triazolam, Whole blood, α-OH Triazolam, α-OH alprazolam

This application note describes the extraction of barbiturate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, benzodiazepines, cocaine and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Tags: Amobarbital, Application Notes, Barbiturates, Butabarbital, Butalbarbital, Clinical, Column, English, GC-MS, Hexobarbital, Pentobarbital, Phenobarbital, Secobarbital, Whole blood

This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and cocaine. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 50 ng/mL (analyte dependant).
Tags: 6-acetylmorphine (MAM), Application Notes, Clinical, Codeine, Column, Dihydrocodeine (DHC), Drugs of Abuse, English, Forensic, GC-MS, Hydrocodone, Hydromorphone, Morphine, Opiates, Oxycodone, Oxymorphone, Whole blood

Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses. This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis. The method achieves high reproducible analyte recoveries from both gelding and filly urine. Protocols for 48-well, 96-well plate and column formats are described.
Tags: 48-well plate, 96-well plate, Anabolic steroids, Application Notes, Column, English, Ethylestrenol, Forensic, LC-MS/MS, Stanozolol, Urine (horse)

This application note describes the extraction of 11-nor-9-carboxy-THC from a urine matrix, prior to GC/MS analysis. Carboxy-THC is the primary metabolite of THC, a key indicator of illicit marijuana usage. In urine, ~80% of the carboxy-THC metabolite is present in the form of its glucuronide metabolite. Therefore, to effectively quantify the THC-COOH, urine is hydrolyzed before extraction. This application note describes optimized extraction of urine samples prepared by either enzymatic or base hydrolysis.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, Drugs of Abuse, English, Forensic, GC-MS, Hydrolysed urine, THC metabolites, THC-COOH, Urine

This application note describes the extraction of Δ9-THC, 11-hydroxy- Δ9-THC and 11-nor-9-carboxy-THC from whole blood matrix, prior to GC/MS analysis. An extremely simple sample pre-treatment and extraction method is employed to extract the parent THC and main metabolites from complex whole blood matrix, delivering high, reproducible analyte recoveries.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, Drugs of Abuse, English, Forensic, GC-MS, THC, THC metabolites, THC-COOH, Whole blood, Δ9-tetrahydrocannabinol (THC)

This application note describes a supported liquid extraction protocol for the extraction of methylisothiazolinone from sun lotion, prior to GC/MS analysis.
Tags: Antimicrobial, Application Notes, Clinical, Column, English, GC-MS, MI (methylisothiazolinone), Methylisothiazolinone, Preservative, Sun Lotion, Sunscreen

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix after sampling via Oral-Eze collection devices, prior to UPLC-MS/MS analysis using ISOLUTE SLE+ supported liquid extraction columns. Estimated LOQs of between 0.005 and 0.75 ng/mL (analyte dependant) are achieved,.
Tags: 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Application Notes, Basic, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Oral fluid , Over the counter pharmaceuticals, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Pharmaceuticals, Phencyclidine (PCP), Saliva, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, α-OH Triazolam, α-OH alprazolam

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to UPLC-MS/MS analysis. The sample preparation is optimised to minimise matrix effects due to the buffers used in the collection device. Estimated LOQs range from 0.1-1 ng/mL for the various analytes.
Tags: 2-Hydroxyethylflurazepam, 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Application Notes, Basic drugs, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Pharmaceuticals, Phencyclidine (PCP), Saliva, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, α-OH Triazolam, α-OH alprazolam

This study suggests that supported liquid extraction using ISOLUTE®SLE+ 96-well extraction plates is a viable alternative to liquid-liquid extraction in a busy clinical laboratory.
Tags: Application Notes, Product Notes

This application note describes the extraction of a suite of NBOMe designer drugs from oral fluid matrix with analysis by laser diode thermal desorption (LDTD)-MS/MS.
Tags: 25B-NBOMe, 25C-NBOMe, 25D-NBOMe, 25E-NBOMe, 25H-NBOMe, 25N-NBOMe, 25T2-NBOMe, Application Notes, Column, Drugs of Abuse, English, Forensic, LDTD-MS/MS, Legal highs, NBOMes, Oral fluid , Saliva

This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to analysis by UPLC-MS/MS. Optimised protocols for 200 uL and 500 uL sample volumes are included.
Tags: 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Amphetamine, Amphetamines, Application Notes, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Cathinones, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Opiates, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phencyclidine (PCP), Saliva, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, α-OH Triazolam, α-OH alprazolam

This application note describes the extraction of phencyclidine (PCP) from urine using supported liquid extraction and subsequent analysis by GC/MS.
Tags: Application Notes, Column, Drugs of Abuse, English, Forensic, GC-MS, Phencyclidine, Phencyclidine (PCP), Urine

This application note describes the extraction of the 'date rape' drug GHB (gamma-hydroxybutyric acid) from urine using supported liquid extraction and subsequent analysis by GC/MS. Both 96-well plate and column formats are included, and demonstrate good analyte recovery and reproducibility, at levels appropriate for determining illicit use of GHB.
Tags: 96-well plate, Anaesthetic, Application Notes, Column, English, Forensic, GC-MS, GHB Gamma hydroxybutyric acid, Urine

This application note describes the extraction of a range of amphetamines and metabolites from urine using supported liquid extraction and subsequent analysis by GC/MS. Prior to extraction, a simple oxidation step is performed to eliminate sympathomimetic compounds such as ephedrine and pseudoephedrine so they do not interfere with quantitation of methamphetamine.
Tags: 96-well plate, Amphetamine, Amphetamines, Application Notes, English, Ephedrine, Forensic, GC-MS, MDA, MDEA, MDMA, Methamphetamine, Pseudoephedrine, Urine

This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, English, Forensic, GC-MS, Oral fluid , Saliva, THC, THC metabolites, THC-COOH, Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction of Butalbarbital, Butabarbital, Amobarbital, Pentobarbital,Secobarbital, Hexobarbital and Phenobarbital from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.
Tags: Amobarbital, Application Notes, Barbiturates, Butabarbital, Butalbarbital, Column, English, Forensic, GC-MS, Hexobarbital, Oral fluid , Pentobarbital, Phenobarbital, Saliva, Secobarbital

This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Quantisal™ (Immunalysis) device, prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 75% with RSDs lower than 10% for all analytes.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, Drugs of Abuse, Forensic, GC-MS, Oral fluid , Saliva, THC metabolites, Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction of barbiturates from oral fluid matrix collected using Quantisal™ collection devices (Immunalysis), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.
Tags: 96-well plate, Amobarbital, Application Notes, Butabarbital, Butalbarbital, Forensic, GC-MS, Hexobarbital, Oral fluid , Pentobarbital, Phenobarbital, Saliva, Secobarbital

This application note describes the extraction of barbiturates from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit(Orasure Technologies), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 7% for all analytes.
Tags: Amobarbital, Application Notes, Barbiturates, Butabarbital, Butalbarbital, Column, Drugs of Abuse, Forensic, Hexobarbital, LC-MS/MS, Oral fluid , Pentobarbital, Phenobarbital, Saliva, Secobarbital

This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to GC/MS analysis.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, Drugs of Abuse, English, Forensic, GC-MS, Oral fluid , Saliva, THC, THC metabolites, THC-COOH

This application note describes the extraction of the anaesthetic agent Propofol from whole blood prior to GC/MS analysis.
Tags: Anaesthetic, Application Notes, Clinical, Column, English, Forensic, GC-MS, Propofol, Whole blood

The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (Immunalysis) oral fluid collection devices. This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 11-nor-9-carboxy- Δ9-tetrahydrocannabivarin (THC-V-COOH), 11-nor-9-carboxy- Δ9tetrahydrocannabinol glucuronide (THC-COOH-g, 11-nor-9-carboxy-Δ9-THC glucuronide, Application Notes, Cannabidiol, Cannabigerol, Column, Drugs of Abuse, English, Forensic, LC-MS/MS, Oral fluid , Saliva, THC, THC metabolites, THC-COOH, Δ9-tetrahydrocannabinol (THC), Δ9-tetrahydrocannabivarin (THCV)

This application note describes a simple but effective method for the extraction of THC and metabolites from whole blood using ISOLUTE SLE+ Supported Liquid Extraction 96-well plates.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 96-well plate, Application Notes, Drugs of Abuse, English, Forensic, LC-MS/MS, THC, THC metabolites, THC-COOH, Whole blood, Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction of neutral and zwitterionic antiepileptic drugs from fortified oral fluid using ISOLUTE® SLE+ in a 96-well plate format.
Tags: 96-well plate, Antiepileptics, Application Notes, Carbamazepine 10,11 epoxide, Clinical, English, Felbamate, Gabapentin, LC-MS/MS, Oral fluid , Oxcarbazepine, Rufinamide, Tigabatine, Vigabatrin

This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE SLE+ in a 96-well plate format.
Tags: 96-well plate, Acidic, Application Notes, Clinical, English, Immunosuppressant, LC-MS/MS, Mycophenolic Acid, Mycophenolic Acid Glucuronide, Serum

This application note describes the simultaneous extraction of THC and its major metabolites, including 11-nor-9-carboxy-Δ9-THC glucuronide, from urine using supported liquid extraction (ISOLUTE® SLE+ in both plate and column formats) prior to analysis by LC-MS/MS.
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 11-nor-9-carboxy-Δ9-THC glucuronide, 96-well plate, Application Notes, Cannabidiol, Cannabinol, Column, Drugs of Abuse, English, Forensic, LC-MS/MS, THC metabolites, Urine, Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction of a range of recreational ecstasy-like drugs, designer stimulants and the hallucinogen 2C-B from urine prior to GC/MS analysis. The method described in this application note may also be suitable for extraction of other related analogs and derivatives of these drug compounds, for example the substituted cathinone class (bathsalts). This application note is optimized for extraction of 1 mL sample volumes.
Tags: 2C-B, 5-APB, 6-APB, Amphetamine, Application Notes, BZP, Column, Designer stimulants, Drugs of Abuse, Forensic, GC-MS, TFMPP, Urine

The ability to therapeutically monitor antiepileptic drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of the drug. A fast and clean extraction method is needed that works in a variety of biological matrices and affords a high throughput workflow. Here ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum and urine with good efficiency. The method was developed using a 96-well plate format to facilitate a high throughput workflow model.
Tags: 96-well plate, Antiepileptics, Application Notes, Carbamazepine 10,11 epoxide, Clinical, English, Felbamate, Gabapentin, LC-MS/MS, Oxcarbazepine, Rufinamide, Serum, Tigabatine, Urine, Vigabatrin

This application note describes effective and efficient protocols optimized for loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs of <10% for all analytes.
Tags: Anhydroecgonine Methyl Ester AEME, Anhydroecgonine methyl ester, Application Notes, Benzoylecgonine, Cocaethylene, Cocaine, Cocaines, Column, Drugs of Abuse, Ecgonine Methyl Ester EME, Ecgonine methyl ester, English, Forensic, GC-MS, Norcocaine, Oral fluid

This application note describes effective and efficient protocols optimized for sample volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs of <10% for all analytes.
Tags: Anhydroecgonine Methyl Ester AEME, Application Notes, Benzoylecgonine, Cocaethylene, Cocaine, Cocaines, Column, Drugs of Abuse, Ecgonine Methyl Ester EME, English, Forensic, GC-MS, Norcocaine, Urine

This application note describes a novel sample cleanup strategy to address the complex matrix effects associated with commercial milk product variables while maintaining adequate analyte recovery and repeatability.
Tags: Application Notes, Column, Di-iso-nonyl phthalate, Dibutyl phthalate, Diethyl phthalate, Dioctyl phthalate, Dipentyl phthalate, English, Food, LC-MS/MS, Milk, Phthalates, Phthalic acid esters, Plasticisers

This application note describes a novel approach to the extraction of acrylamide from potato chips (crisps) using ISOLUTE SLE+ supported liquid extraction columns, with analysis by LC-MS/MS.
Tags: Acrylamide, Application Notes, Column, English, Food, Food contaminant, LC-MS/MS, Potato chips (crisps)

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection. The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb in ground coffee (solid) or 125 ppb in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.
Tags: Acrylamide, Application Notes, Coffee, Column, English, Food, Food contaminant, LC-MS/MS

This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual (Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.
Tags: 96-well plate, Application Notes, English, Forensic, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018, JWH-018-(5-hydroxypentyl) , JWH-018-N-5-(pentanoic acid), JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250, JWH-250-N-(5-hydroxypentyl), LC-MS/MS, Synthetic Cannabinoids (SPICE), TECAN, UR-144 , UR-144-(5-hydroxypentyl) , UR-144-(pentanoic acid) , UR144-(5-chloropentyl) , Urine, XLR-11

This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats. SPICE, oral fluid, Intercept Orasure, LC-MSMS, SLE
Tags: 96-well plate, Application Notes, Column, English, Forensic, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018-N-5-(pentanoic acid), JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250, JWH-250-N-(5-hydroxypentyl), LC-MS/MS, Oral fluid , Synthetic Cannabinoids (SPICE), UR-144 , UR-144-(5-hydroxypentyl) , UR-144-(pentanoic acid) , UR144-(5-chloropentyl) , XLR-11

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of nicotine, cotinine, 3-OH-cotinine, nornicotine, norcotinine and anabasine from urine, serum, plasma and whole blood using ISOLUTE® SLE+ plates with LC-MS/MS detection. nicotine cotinine nornicotine norcotinine 3-OH cotinine anabasine smoker
Tags: 3-OH-cotinine, Anabasine, Application Notes, Clinical, Cotinine, LC-MS/MS, Nicotine, Nicotine metabolites, Norcotinine, Nornicotine, Plasma, Serum, Urine, Whole blood

This application note describes effective and efficient protocols optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <10% for all analytes.
Tags: AM-2201, Application Notes, Column, English, Forensic, GC-MS, Hydrolysed urine, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018, JWH-018-(5-hydroxypentyl) , JWH-073, JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250-N-(5-hydroxypentyl), Synthetic Cannabinoids (SPICE), UR-144

This application note describes the extraction of a range of synthetic cannabinoids and metabolites from oral fluid collected using the Quantisal™ Oral Fluid Collection Device prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for loading volumes of either 400 μL or 1 mL of matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs <10% for all analytes.
Tags: AM-2201, Application Notes, Column, English, Forensic, GC-MS, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018-(5-hydroxypentyl) , JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250-N-(5-hydroxypentyl), Oral fluid , Saliva, Synthetic Cannabinoids (SPICE), UR-144

This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes. Cortisol, Saliva, SLE, Supported Liquid Extraction, ISOLUTE, Clinical, Steroid Hormones, Stress
Tags: Application Notes, Clinical, Cortisol, LC-MS/MS, Saliva, Steroid Hormones

Cortisol is a steroid hormone that in urine can be used to diagnose hyper or hypo cortisol diseases such as Cushing’s Syndrome. This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of urinary cortisol levels. Analyte recoveries achieved using this method ranged from 99-101% with RSDs below 5% for all analytes. Cortisol, Steroid hormone, Cushing's Syndrome, Cushing's Disease, Clinical, SLE, Supported Liquid Extraction, Stress,
Tags: Application Notes, Clinical, Cortisol, LC-MS/MS, Steroid Hormones, Urine

‘Bath salts’ is the street name for a family of designer drugs chemically similar to cathinones that give the user similar effects to amphetamines. The abuse of these drugs is on the increase and regulation against their use and supply has now been implemented in the EU and North America. Analyte recoveries achieved using this method to extract bath salts from urine ranged from 87-99% with RSDs below 10% for all analytes. Bath Salts, MCAT, Substitued Cathinones, SLE, SPE, SLE+, ISOLUTE, Agilent, Waters, Supported Liquid Extraction, Forensic, Drugs of Abuse, Regulation
Tags: Application Notes, Bath Salts, Butylone, Ethylone, Forensic, GC-MS, MDPV, Mephedrone, Methamphetamine, Methedrone, Methylone, Naphyrone, Urine

This application note describes the extraction of a range of synthetic cannabinoids and metabolites from whole blood prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for extraction of 800 μL of pre-treated matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs lower than 10% for all analytes.
Tags: AM-2201, Application Notes, Column, English, Forensic, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018-(5-hydroxypentyl) , JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250-N-(5-hydroxypentyl), LC-MS/MS, Synthetic Cannabinoids (SPICE), UR-144 , Whole blood

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from non- hydrolyzed urine prior to LC-MS/MS analysis. drugs of abuse, sle, multiclass
Tags: 2-Hydroxyethylflurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Application Notes, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, Hydroxyalprazolam, Hydroxytriazolam, Ketamine, LC-MS/MS, MDA, MDEA, MDMA, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opioid, Oxazepam, Oxymorphone, RapidTrace, TECAN, Temazepam, Triazolam, Urine, Zaleplone, Zolpidem, Zopiclone, z-drugs

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of various drugs of abuse from hydrolyzed urine prior to LC-MS/MS analysis. drugs of abuse, sle, multiclass
Tags: 2-Hydroxyethylflurazepam, 7-Aminoclonazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Anhydroecgonine methyl ester, Application Notes, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clonazepam, Cocaethylene, Cocaine, Cocaines, Codeine, Column, Diazepam, Ecgonine methyl ester, Estazolam, Fentanyl, Flunitrazepam, Hydrolysed urine, Hydroxyalprazolam, Hydroxytriazolam, Ketamine, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Norcocaine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opioid, Oxazepam, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, z-drugs

This application note describes effective and efficient protocols for extraction of cocaine and its major metabolites optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <7% for all analytes.
Tags: Anhydroecgonine Methyl Ester AEME, Application Notes, Benzoylecgonine, Cocaethylene, Cocaine, Cocaines, Column, Drugs of Abuse, Ecgonine Methyl Ester EME, English, Forensic, GC-MS, Hydrolysed urine, Norcocaine

This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant amphetamines followed by derivatization to optimize for GC-MS analysis. Analyte recoveries achieved using this method ranged from 99-104% with RSDs below 10% for all analytes. Amphetamines, SLE, Supported Liquid Extraction, SLE+, ISOLUTE, Urine, GC, GC-MS,
Tags: Amphetamine, Application Notes, Column, Drugs of Abuse, Forensic, GC-MS, MDEA, MDMA, Methamphetamine, Urine

This application note describes the extraction and quantitation of Cannabimimetic Naphthoylindoles (Synthetic Cannabinoids) and their metabolites (JWH Series) from various matrices using Supported Liquid Extraction (SLE). Synthetic Cannabinoids or SPICE as they are commonly known have become an increasing problem as one of the newest forms of illicit drugs being consumed today. These compounds bind to the cannabinoid receptors in mammals triggering similar euphoric symptoms as Tetrahydrocannabinoids (THC). Currently robust and fast analytical methods of analysis are required to aid in the screening and detection of this growing class of compounds. The recoveries obtained for the synthetic cannabinoids parent and metabolites ranged from 70-98 %. Supported Liquid Extraction, SLE, SLE+, SPICE, Synthetic Cannabinoids, JWH, metabolites,
Tags: Application Notes, Column, Forensic, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018-N-5-(pentanoic acid), JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250, JWH-250-N-(5-hydroxypentyl), JWH019, LC-MS/MS, Plasma, Synthetic Cannabinoids (SPICE), Urine, Whole blood

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 67-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.
Tags: Anhydroecgonine Methyl Ester AEME, Application Notes, Benzoylecgonine, Butylone, Cocaines, Column, Ecgonine Methyl Ester EME, Forensic, LC-MS/MS, Norcocaine, Urine

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (hydrolysed urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 82-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.
Tags: Anhydroecgonine Methyl Ester AEME, Application Notes, Benzoylecgonine, Butylone, Cocaines, Column, Ecgonine Methyl Ester EME, Forensic, Hydrolysed urine, LC-MS/MS, Norcocaine

This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites opiates, sle, urine, GC-MS, forensic, drugs of abuse, DOA, UCT, agilent
Tags: 6-acetylmorphine (MAM), Application Notes, Codeine, Column, Dihydrocodeine (DHC), Drugs of Abuse, Forensic, GC-MS, Hydrocodone, Hydromorphone, Methadone, Morphine, Oxycodone, Oxymorphone, Urine

The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed and extracted to detect the drugs using Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation. LOQs for recovered drugs ranged from 10 ng/mL to 0.5 ng/mL with recoveries above 80% and RSDs <10% SLE, Supported Liquid Extraction, Pain Mangement, Hydrolysis, Urine, Buprenorphine, Oxycodone, Temazepam,
Tags: Alprazolam, Application Notes, Benzodiazepines, Buprenorphine, Clinical, Clonazepam, Codeine, Diazepam, Drugs of Abuse, English, Fentanyl, Flunitrazepam, Hydromorphone, LC-MS/MS, Meperidine, Methadone, Methamphetamine, Morphine, Naloxone, Naltrexone, Nitrazepam, Oxycodone, Pain Management, Temazepam, Urine

This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%. Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,
Tags: Androstenedione, Application Notes, Clinical, LC-MS/MS, Serum, Steroid Hormones, Testosterone

Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%. Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,
Tags: Application Notes, Clinical, Cyclosporin A, Everolimus, Immunosuppressant, LC-MS/MS, Sacrolimus, Sirolimus, Tacrolimus, Whole blood

Vitamin D deficiency can result in a variety of health issues such as osteoporosis, liver and kidney problems, it is often associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D monitoring is on the rise and of significant clinical relevance. This method describes the use of ISOLUTE SLE+ plates for the efficient and simple extraction of the vitamin D metabolites 25-OH vitamin D2 and 25-OH vitamin D3. Incorporated into the procedure is an integral protein binding disruption step which maximizes analyte recovery and eliminates the need for any offline protein disruption. This method has been internally validated using DEQAS approved serum samples and deuterated internal standards the results obtained are better than that required for criteria for acceptable performance for DEQAS approval. All recoveries were consistently greater than 90% with all RSDs < 10% at LOQs of 1-3.75 ng/mL. Vitamin, Vitamin D, VitaminD, D, Clinical, SLE+, SLE, ISOLUTE, Metabolites, Supported Liquid Extraction, 25 Hydroxy Vitamin D,
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 96-well plate, Application Notes, Clinical, English, LC-MS/MS, Serum, Vitamin D

Benzodiazepines are psychoactive drugs used to treat various conditions including anxiety, insomnia, seizures and alcohol withdrawal. Long-term use can cause physical dependence and withdrawal upon cessation of use, due to this their application as medication has to be monitored and measured. This application note details an optimized extraction protocol for the use of ISOLUTE SLE+ supported liquid 1 mL sample volume columns to extract a range of benzodiazepines from human urine. This simplified and efficient extraction method has analyte recoveries from 90% with LOQs as low as 40 ng/mL. The application has also been performed with halogenated and non-halogenated extraction solvents in the interests of providing for green chemistry. Drugs of abuse, DOA, Benzodiazepines, SLE, SLE+, Supported Liquid Extraction, Green Chemistry,
Tags: 2-Hydroxyethylflurazepam, 4-Hydroxynordiazepam, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 7-Aminonitrazepam, Alprazolam, Application Notes, Benzodiazepines, Bromazepam, Clonazepam, Demethylflunitrazepam, Desalkylflurazepam, Diazepam, Estazolam, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydroxyalprazolam, Hydroxymidazolam, Hydroxytriazolam, Midazolam, Nitrazepam, Nordiazepam, Oxazepam, Prazepam, Temazepam, Triazolam, Urine

This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LODs as low as 500 pg/mL. Opiates, SLE, SLE+, Supported Liquid Extraction, Urine, Oasis, Waters, SPE, Solid Phase Extraction, Sample Prep, quick, easy, DOA, Drugs of Abuse
Tags: 6-acetylmorphine (MAM), Application Notes, Codeine, Column, Dihydrocodeine (DHC), EDDP, Forensic, Hydrocodone, Hydromorphone, LC-MS/MS, Methadone, Morphine, Opiates, Oxycodone, Oxymorphone, Urine

Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal. Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011
Tags: Application Notes, Aqueous, Biological fluids, Column, LC-MS/MS, Plasma, RapidTrace, Serum, Urine, Whole blood

Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from whole blood prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from whole blood allowing for mass spectral detection of the target analytes at a concentration of 200 ng/mL or less. Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Whole Blood, Clinical, LCMS, Sample Prep
Tags: Application Notes, LC-MS/MS, Retinol, Vitamin A (Retinol), Vitamin E (Tocopherol), Vitamins, Whole blood, a-Tocopherol, β-Carotene

Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from human serum prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from serum allowing for mass spectral detection of the target analytes at a concentration of 100 ng/mL or less. Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Serum, Clinical, LCMS, Sample Prep
Tags: Application Notes, Clinical, LC-MS/MS, Retinol, Vitamin A (Retinol), Vitamin E (Tocopherol), Vitamins, a-Tocopherol, β-Carotene

This method describes the use of ISOLUTE SLE+ supported liquid extraction 1 mL sample volume columns to extract a range of barbiturates from human urine. The analysis of these analytes was carried out by GC-MS. This simplified and efficient extraction method has significant analyte recoveries ranging from 103-108% with LOQs of 10 ng/mL and RSDs <10%. Barbs, Barbiturates, SLE, SLE+, ISOLUTE, GCMS, DoA, Forensic, Work Place Drug Testing, Supported Liquid Extraction, GC
Tags: Amobarbital, Application Notes, Barbiturates, Butabarbital, Butalbarbital, GC-MS, Phenobarbital, Secobarbital, Urine

Benzodiazepines are psychoactive drugs used to treat various conditions including anxiety, insomnia, seizures and alcohol withdrawal. Long-term use can cause physical dependence and withdrawal upon cessation of use, due to this their application as medication has to be monitored and measured. This application note details an optimized extraction protocol for the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of benzodiazepines from human urine. This simplified and efficient extraction method has analyte recoveries from 85% with LOQs as low as 100 pg/mL. SLE+, SUPPORTED LIQUID EXTRACTION, BENZOS, BENZODIAZEPINES, URINE,
Tags: 2-Hydroxyethylflurazepam, Alprazolam, Application Notes, Benzodiazepines, Bromazepam, Clinical, Demethylflunitrazepam, Desalkylflurazepam, Diazepam, Estazolam, Flunitrazepam, Hydroxyalprazolam, Hydroxymidazolam, LC-MS/MS, Midazolam, Nitrazepam, Nordiazepam, Oxazepam, Temazepam, Triazolam, Urine

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis. SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
Tags: Application Notes, Aqueous, Biological fluids, Column, Plasma, Post-Mortem fluids, Urine, Water, Whole blood

This method describes the use of ISOLUTE SLE+ in column format for the extraction of a range of amphetamines. This method is effective for sample volumes of 500μL with analyte recoveries > 95%. Amphetamines, ISOLUTE, DOA, DRUGS OF ABUSE, SLE+, DRUG SCREEN
Tags: Amphetamine, Amphetamines, Application Notes, Ephedrine, Forensic, LC-MS/MS, MDA, MDEA, MDMA, Methamphetamine, Urine

This method describes the use of ISOLUTE SLE+ in 96 well plate format for the extraction of a range of amphetamines. This procedure is effective with sample volumes of 100 μL for analyte recoveries > 90%. For larger volume (500 μL) samples, see AN746. amphetamines, AN742, ISOLUTE, SLE+, DOA, DRUGS OF ABUSE, FORENSIC
Tags: Amphetamine, Amphetamines, Application Notes, Ephedrine, Forensic, LC-MS/MS, MDA, MDEA, MDMA, Methamphetamine, Urine

This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL. ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL
Tags: 11-deoxycortisol, 17-alpha-hydroxyprogesterone, 21-deoxycortisol, Aldosterone, Androstenedione, Androsterone, Application Notes, Clinical, Dehydroepiandrosterone (DHEA), LC-MS/MS, Plasma, Progesterone, Steroid Hormones, Testosterone

This application note describes the extraction of THC and it’s metabolites from plasma using ISOLUTE SLE+ supported liquid extraction plates followed by UPLC-MS/MS analysis.
Tags: Application Notes

This application note describes the extraction of Tamoxifen and metabolites from plasma using ISOLUTE SLE+ supported liquid extraction plates followed by LC-MS/MS analysis.
Tags: Application Notes

This application note describes the extraction of Tamoxifen and metabolites from urine using ISOLUTE SLE+ supported liquid extraction plates followed by LC-MS/MS analysis. ISOLUTE, SLE, SLE+, Tamoxifen, Hormones, Oestrogen
Tags: Application Notes, Clinical, LC-MS/MS

This application note describes the extraction of THC and metabolites from urine using ISOLUTE SLE+ columns or plates. The most prevalent marker in biological samples taken from cannabis abusers is 11-nor-9-carboxy-Δ9-THC. Here we demonstrate a supported liquid extraction procedure for 11-nor-9-carboxy-Δ9-THC.
Tags: Application Notes, Column, Drugs of Abuse, Forensic, LC-MS/MS, THC-COOH, Urine

This Application Note highlights the use of supported-liquid Extraction to isolate three common NSAIDs, Sulindac, Flurbiprofen and Ibuprofen, from human plasma. Biotage, Ostro, Liquid liquid extraction, supported liquid extraction, Extrelute
Tags: Application Notes, Clinical, Flurbiprofen, Ibuprofen, LC-MS/MS, NSAIDs, Plasma, Sulindac

This Application Note highlights the use of ISOLUTE® SLE+ supported-liquid extraction plates for the extraction of corticosteroids from human plasma, specifically Triamcinolone, Prednisolone, Hydrocortisone, Prednisone, Betamethasone, Cortisone, Dexamethasone, Flumethasone, Corticosterone, Beclomethasone, Triamcinolone, Fluocinolone, Budesonide 1, Budesonide 2 and 5-Pregnen-3β−ol-20-one.
Tags: 5-pregnen-3β-ol-20-one, Application Notes, Beclomethasone, Betamethasone, Budesonide Structural Isomer 1, Budesonide Structural Isomer 2, Clinical, Corticosteroids, Corticosterone, Cortisone, Dexamethasone, Flumethasone, Fluocinolone Acetonide, Hydrocortisone, LC-MS/MS, Plasma, Prednisolone, Prednisone, Triamcinolone, Triamcinolone Acetonide

This Chemistry Data Sheet describes the use of ISOLUTE SLE+ Supported Liquid Extraction Plates for the isolation of acidic, basic and neutral drugs from biological fluids.
Tags: Application Notes, Product Notes

This Application Note describes the development of an automated procedure for high throughput supported-liquid extraction of three tricyclic antidepressant drugs from human plasma, using the ISOLUTE® SLE+ Supported-liquid Extraction Plate. Analyte recovery, along with the speed and efficiency are compared to traditional LLE. Biotage, Extralute, Ostro, supported liquid extraction, liquid liquid extraction,
Tags: Application Notes, Clinical, Imipramine, LC-MS/MS, Nortryptiline, Plasma, Tricyclic Antidepressant, Trimipramine

In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs

At Chiba University’s Graduate School of Medicine and its affiliated hospital, researchers are developing an LC-MS/MS method for vitamin D analysis using ISOLUTE® SLE+ and other Biotage products. We interviewed Professor Mamoru Satoh at the Disease Proteomics Center and Mr. Takashi Ishige from the Inspection Department at the affiliated hospital.
Tags: Customer Cases

At the Forensic Medicine Lab at Toho University, researchers use ISOLUTE® SLE+ columns from Biotage. When dealing with samples that easily form emulsions like urine or blood, it allows researchers to use the established liquid-liquid extraction technique, saving significant amount of time on analysis. We spoke with the Head of the Forensic Medicine Lab Professor Masaru Terada.
Tags: Customer Cases

User report: ISOLUTE SLE+. Shin Nippon Biomedical Laboratories, Ltd. use ISOLUTE® SLE+ to prepare biological samples for pharmacokinetic testing as part of a drug discovery and development program. We had the chance to speak with Mr. Mitsuhiko Kawabata and Ms. Akiko Toda of the pharmacokinetics group.
Tags: Customer Cases

This guide describes how to develop supported liquid extraction methods using ISOLUTE® SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted
Tags: 48-well plate, 96-well plate, Column, English, Manuals & User Guides

This method selection guide is designed to minimize the choices required to optimize the Supported Liquid Extraction method. The buffer and solvent suggestions provide a range of pHs and solvent polarities for acidic, neutral and basic drugs of varying pKa and logP values. The selections are based on extensive work in Biotage’s R&D Laboratories.
Tags: English, Manuals & User Guides

•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides

This method card provides recommended conditions for using ISOLUTE® SLE+ Supported Liquid Extraction Plates and Columns.
Tags: Manuals & User Guides, Product Notes

laboratoriekemikalie inte för terapeutisk eller diagnostisk användning
Tags: Material Safety Data Sheets, Swedish

Safety Data Sheet
Tags: English, Material Safety Data Sheets

This poster describes an improved workflow for the analysis of drugs of abuse from hair. TIAFT 2018, Ghent, Belgium
Tags: 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), EDDP, English, Estazolam, Extrahera, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hair, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, Lysera, Lysergic acid diethylamide (LSD), MDA, MDEA, MDMA, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, PCP, Posters, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam

This poster compares sample preparation options (solid phase extraction using EVOLUTE EXPRESS ABN, and supported liquid extraction using ISOLUTE SLE+) for the extraction of a panel of endogenous steroids from serum. MSACL NA 2018, Palm Springs, CA ASMS 2018, San Diego, CA
Tags: 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Steroid Hormones, Steroid metabolite

Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5 HIAA) are biomarkers for neuroblastoma and catecholamine-secreting tumors. This poster presents optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Sample preparation protocols using solid phase extraction (EVOLUTE EXPRESS ABN and AX) and supported liquid extraction (ISOLUTE SLE+) are presented. MSACL NA 2018 Palm Springs, CA
Tags: 5-hydroxyindoleacetic acid (5-HIAA) , 96-well plate, Acidic catecholamine metabolites, Catecholamines, Clinical, English, Forensic, Homovanillic acid (HVA), LC-MS/MS, Plasma, Posters, Vanillylmandelic acid (VMA)

This poster presents a high sensitivity method for extraction and analysis of estrogens in human plasma. Sample preparation utilizes Biotage ISOLUTE SLE+ plates, providing high analyte recovery and low matrix effects. MSACL 2018, Palm Spings, CA
Tags: 96-well plate, Clinical, English, Estradiol, Estriol, Estrogens, Estrone, LC-MS/MS, Plasma, Posters

This poster discusses the potential for a single extraction protocol for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis. SOFT 2017, Boca Raton also presented at SFTA, Marseille, France, 2018
Tags: Amphetamines, Benzodiazepines, Cannabinoid, Cathinones, Clinical, Cocaines, Column, Drugs of Abuse, English, Extrahera, Forensic, LC-MS/MS, Opiates, Posters, THC metabolites, Whole blood, z-drugs

This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids. SOFT 2017, Boca Raton
Tags: Amphetamines, Barbiturates, Benzodiazepines, Cannabinoid, Cathinones, Clinical, Cocaines, Column, English, Forensic, GC-MS, Opiates, Oral fluid , Posters, Saliva, Synthetic Cannabinoids (SPICE)

This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels. Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc). ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

Over the past decade, the need for non-invasive drug screening that that precludes sample adulteration has become attractive. As a result, detection using oral fluid devices for Drugs of Abuse (DOA) has come to the vanguard of the scientific community. The use of Supported Liquid Extraction (ISOLUTE® SLE+) prior to LC/MS or GC/MS can improve sample cleanliness without forfeiting sample detection within a diverse panel of DOAs. Here, we demonstrate the effects of altering elution solvent polarity and pH for sample pretreatment upon the simultaneous recovery of 34 compounds comprised of opioids, benzodiazepines, and stimulants to directly measure the effects of the oral fluid buffer, OraSure™, upon extraction and signal intensity at presumed LOQs. ASMS 2017
Tags: 6-acetylmorphine (MAM), 7-Aminoclonazepam, Alprazolam, Amphetamine, Benzodiazepines, Benzoylecgonine, Buprenorphine, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Column, Cotinine, Diazepam, EDDP, English, Forensic, Hydrocodone, Hydromorphone, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Meperidine, Methadone, Methamphetamine, Midazolam, Morphine, Norbuprenorphine, Nordiazepam, Opiates, Opioid, Oral fluid , Oxazepam, Phencyclidine, Phencyclidine (PCP), Posters, Stimulants, Temazepam, Triazolam, α-OH Triazolam, α-OH alprazolam

Hair is a useful matrix for drug analysis, as it can be used to show historical drug use. Parent drugs are often present in high concentrations, and the matrix is stable over time. This poster describes the use of the Biotage® Lysera to pulverise and extract hair samples, with ISOLUTE® SLE+ supported liquid extraction columns to clean up the samples prior to analysis. Limits of quantitation set by the Society of Hair Testing for amphetamines are achievable with the described method (0.2 ng/mg). SoHT 2017, Cardiff UK
Tags: Amphetamine, Amphetamines, Bead Ruptor, Column, Drugs of Abuse, English, Forensic, GC-MS, Hair, MDA, MDEA, MDMA, Methamphetamine, Posters

This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination. TIAFT 2016 SOFT 2016
Tags: 2-Hydroxyethylflurazepam, 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Bromazepam, Buprenorphine, Clinical, Clonazepam, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Extrahera, Fentanyl, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opiates, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, Temazepam, Triazolam, Urine, Whole blood, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam

This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility. MSACL 2016
Tags: 11-deoxycortisol, 11-keto-androsterone, 11-oxoetiocholanolone, 11-β-hydroxyandrosterone, 11β-hydroxyetiocholanolone, 2-OH-estrone, 2-methoxyestrone, 3α-androstanediol, 3β-androstanediol, 4-OH-estrone, 4-methoxyestrone, 96-well plate, Allo-THB, Androsterone, Clinical, Corticosterone, DHEA (dehydroepiandrosterone, Dehydroepiandrosterone (DHEA), English, Etiocholanolone, Extrahera, Hydrolysed urine, Posters, Steroid Hormones, Steroid metabolite, THB, THF, Testosterone, α-THF

The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS. This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS. MSACL 2016
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetyl codeine, 96-well plate, Acidic, Alprazolam, Amphetamine, Barbiturates, Basic, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Clinical, Clonazepam, Codeine, Dextromethorphan, Diazepam, Drugs of Abuse, EDDP, English, Fentanyl, Flunitrazepam, Forensic, Hydrocodone, Hydrolysed urine, Hydromorphone, LC-MS/MS, MDEA, Morphine, Naltrexone, Neutral, Nitrazepam, Norbuprenorphine, Norcodeine, Norfentanyl, Opiates, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Pentazocine, Pentobarbital, Phenobarbital, Posters, Propoxyphene, Secobarbital, THC, THC metabolites, THC-COOH, Temazepam, Δ9-tetrahydrocannabinol (THC)

Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+). MSACL 2016
Tags: 96-well plate, Buprenorphine, Clinical, Column, English, Forensic, LC-MS/MS, Norbuprenorphine, Oral fluid , Pain Management, Posters, Saliva, Synthetic urine, Urine, Whole blood

This poster summarizes various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal. MSACL 2016
Tags: 96-well plate, Acidic, Clinical, English, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Posters, Serum

This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016
Tags: 2-Hydroxyethylflurazepam, 2C-B, 5-APB, 6-APB, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amobarbital, Amphetamine, Anhydroecgonine Methyl Ester AEME, BZP, Benzoylecgonine, Bromazepam, Butabarbital, Butalbarbital, Butylone, Clinical, Clonazepam, Cocaethylene, Cocaine, Cocaines, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, Ecgonine Methyl Ester EME, English, Estazolam, Ethylone, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hexobarbital, Hydrocodone, Hydromorphone, Lorazepam, MDA, MDEA, MDMA, MDPV, Mephedrone, Methamphetamine, Methcathinone, Methedrone, Methylone, Midazolam, Morphine, Naphyrone, Nitrazepam, Nordiazepam, Oxazepam, Oxycodone, Oxymorphone, Pentobarbital, Phenobarbital, Posters, Secobarbital, TFMPP, Temazepam, Triazolam, Whole blood, mCPP, pMMA, α-OH Triazolam, α-OH alprazolam

This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared. MSACL EU 2015
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, Extrahera, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster describes a simple supported liquid extraction (SLE) method for extraction of THC and metabolites from whole blood and urine, suitable for GC-MS analysis. SOFT 2015
Tags: 11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH), 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Column, Drugs of Abuse, English, Forensic, GC-MS, Posters, THC, THC-COOH, Urine, Whole blood, Δ9-tetrahydrocannabinol (THC)

This poster describes a simple supported liquid extraction (SLE) method for extraction of THC and metabolites from oral fluid, suitable for analysis by GC-MS. TIAFT 2015
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Column, Drugs of Abuse, English, Forensic, Oral fluid , Posters, THC, THC-COOH, Δ9-tetrahydrocannabinol (THC)

This poster demonstrates a simple supported liquid extraction procedure for the extraction of propofol from whole blood suitable for GC-MS analysis. In addition, phospholipid profiles in the extracts were investigated using LC-MS/MS, and showed no breakthrough of these blood components, suggesting the procedure is appropriate for use with LC-MS/MS based analytical procedures. TIAFT 2015
Tags: Anaesthetic, Column, English, Forensic, GC-MS, LC-MS/MS, Posters, Propofol, Whole blood

This poster demonstrates the extraction of a range of drugs of abuse from oral fluid collection devices using supported liquid extraction suitable for UPLC-MS/MS analysis. Unlike some sample preparation techniques, SLE allows for the simultaneous extraction of cross-functional analytes in a single extraction protocol without forfeiting extract cleanliness. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine. TIAFT 2015
Tags: 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Cathinones, Clonazepam, Cocaine, Cocaines, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, LC-MS/MS, Lorazepam, MDA, MDMA, Mephedrone, Methadone, Midazolam, Morphine, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine (PCP), Posters, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, α-OH Triazolam, α-OH alprazolam

Methylisothiazolinone (MI) is used in a variety of personal care products, such as sunscreens, lotions, cosmetics. MI is a cytotoxin and as a result there is concern because of sensitization and allergic reactions as well as cell and nerve damage. A percentage of the population is at risk from contact dermatitis when exposed to this compound at sufficient concentrations. This poster describes the use of ISOLUTE SLE+ for extraction of MI from sunscreen. ASMS 2015
Tags: Antimicrobial, Clinical, Column, Environmental, GC-MS, MI (methylisothiazolinone), Methylisothiazolinone, Posters, Preservative, Sun Lotion, Sunscreen

This poster describes an evalution of Methylisothiazolinone (MI) extraction from sunscreen using supported liquid extraction prior to GC/MS analysis MSACL 2015, San Diego
Tags: Antimicrobial, Clinical, Column, English, Environmental, GC-MS, MI (methylisothiazolinone), Methylisothiazolinone, Posters, Sun Lotion, Sunscreen

This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster describes the supported liquid extraction method development process for the extraction of the NBOMes group of novel psychoactive substances from oral fluid, for analysis using GC-MS. both sample pre-treatment and elution solvent were optimised, and the final method compared to traditional LLE and SPE procedures. AAFS 2015
Tags: 25B-NBOMe, 25C-NBOMe, 25D-NBOMe, 25E-NBOMe, 25H-NBOMe, 25I-NBOMe, 25N-NBOMe, 25T2-NBOMe, Column, Drugs of Abuse, English, Forensic, GC-MS, NBOMes, Oral fluid , Posters

This poster examines the use of ISOLUTE SLE+ columns as an alternative to SPE for the extraction and clean up of hair samples containing drugs of abuse. SLE was found to be a simple, faster alternative to SPE for this type of analysis. AAFS 2015
Tags: 6-acetylmorphine (MAM), Benzoylecgonine, Cocaine, Cocaines, Codeine, Column, Drugs of Abuse, English, Forensic, GC-MS, Hair, Methadone, Morphine, Posters

This poster describes simple, effecive methods optimised for extraction of THC and metabolites from popular oral fluid collection devices, optimised for GC-MS analysis. SOFT 2014
Tags: Column, Drugs of Abuse, English, Forensic, GC-MS, Oral fluid , Posters, THC metabolites, THC-COOH, Δ9-tetrahydrocannabinol (THC)

This poster describes a simple and effective method for extraction of the anaesthetic propofol from whole blood using supported liquid extraction, suitable for GC-MS analysis. SOFT 2014
Tags: Anaesthetic, Column, English, Forensic, GC-MS, Posters, Propofol, Whole blood

This poster demonstrates the extraction of a range of drugs of abuse from oral fluid, collected with common collection devices, prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine. SOFT 2014
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Cathinones, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opiates, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, Saliva, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam

This poster describes the extraction of barbiturates from oral fluid collected using common collection devices using ISOLUTE SLE+ Supported Liquid Extraction columns, optimised for GC-MS analysis. SOFT 2014
Tags: Amobarbital, Barbiturates, Butabarbital, Butalbarbital, Column, English, Forensic, GC-MS, Hexobarbital, Oral fluid , Pentobarbital, Phenobarbital, Posters, Secobarbital

This poster describes a supported liquid extraction method for extraction of a class of novel psychoactive substances (NBOMes) with analysis using laser diode thermal desorption (LDTD) MS/MS. Samples are oral fluid collected using Salivettes. TAMS 2014
Tags: 25B-NBOMe, 25C-NBOMe, 25D-NBOMe, 25E-NBOMe, 25H-NBOMe, 25I-NBOMe, 25N-NBOMe, 25T2-NBOMe, 96-well plate, Drugs of Abuse, English, Forensic, LDTD-MS/MS, Legal highs, NBOMes, Oral fluid , Phenethylamines, Posters

This poster describes the development and optimisation of the dual extraction method for Vitamin D metabolites using ISOLUTE SLE+.
Tags: 1, 25 Dihydroxyvitamin D, 1a, 25-dihydroxy-vitamin D, 1α, 25-dihydroxy-vitamin D, 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster presents a novel method for the simultaneous extraction, derivatization and subsequent detection of both the traditional 25-hydroxy and the biologically active 1α,25-dihydroxy vitamin D metabolites in serum. ASMS2014
Tags: 1, 25 Dihydroxyvitamin D, 1a, 25-dihydroxy-vitamin D, 1α, 25-dihydroxy-vitamin D, 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster describes the extraction of a range of drugs of abuse (including barbiturates, THC and metabolites, benzodiazepines, z drugs, amphetamines,cathinones, opiates, cocaine, buprenorphine, PCP, fentanyl and ketamine) from oral fluid using supported liquid extraction (ISOLUTE SLE+) columns prior to GC-MS and LC-MS/MS analysis. ASMS 2014
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Amphetamine, Amphetamines, Barbiturates, Benzodiazepines, Benzoylecgonine, Bromazepam, Buprenorphine, Clonazepam, Cocaine, Codeine, Column, Designer stimulants, Diazepam, Dihydrocodeine (DHC), EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydrocodone, Hydromorphone, Hydroxyalprazolam, Hydroxytriazolam, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, THC metabolites, Temazepam, Triazolam, Zaleplone, Zolpidem, Zopiclone

This poster demonstrates a novel, sensitive, cost effective and rugged method for the analysis of acrylamide in coffee and potato chips (crisps) using supported liquid extraction prior to LC-MS/MS analysis. It demonstrates extraction from coffee down to a concentration of 1 ng/mL and in potato chips to a level of 10 ng/g (ppb). ASMS 2014
Tags: Acrylamide, Coffee, Column, English, Food, Food contaminant, LC-MS/MS, Posters, Potato chips (crisps)

This poster describes the extraction of the commonly used immunosuppressant drug mycophenolic acid and its glucuronide metabolite from serum using ISOLUTE SLE+ supported liquid extraction plates. ASMS 2014
Tags: 96-well plate, Clinical, Immunosuppressant, LC-MS/MS, Mycophenolic Acid, Mycophenolic Acid Glucuronide, Posters, Serum

In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract antiepileptic drugs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96-well plate format to facilitate a high throughput workflow model. ASMS 2014
Tags: 96-well plate, Antiepileptics, Carbamazepine 10,11 epoxide, Clinical, English, Felbamate, Gabapentin, LC-MS/MS, Oral fluid , Oxcarbazepine, Posters, Rufinamide, Saliva, Serum, Tigabatine, Urine, Vigabatrin

This poster describes the benefits of supported liquid extraction and highlights its use in removal of endogenous matrix components that cause ion suppression/enhancement (matrix effects) in LC-MS/MS analyses. HPLC 2014
Tags: 96-well plate, Clinical, Column, English, Forensic, LC-MS/MS, Pharma, Plasma, Posters, Urine, Whole blood

This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches. HPLC2014
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters

Immunosuppressant drugs are instrumental in preventing organ and tissue rejection in patients undergoing transplant surgery. Mycophenolic Acid is a common immunosuppressant drug used in patient transplant therapy. The ability to monitor the trough levels in patients to evaluate dosing is important for the administering of the drug. The free drug and its glucuronidated metabolite can be found in patient serum. The ability to extract the free drug and metabolite in patient serum is supported by using a fast and efficient sample preparation extraction method called Supported Liquid Extraction (ISOLUTE SLE+). The free drug and metabolite can be recovered from serum using ISOLUTE SLE+ with high enough efficiency to allow for quantitation at target trough levels. MSACL 2014
Tags: 96-well plate, Clinical, English, Immunosuppressant, LC-MS/MS, Mycophenolic Acid, Mycophenolic Acid Glucuronide, Posters, Serum

Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices. MSACL 2014
Tags: 6-acetyl codeine, 6-acetylmorphine (MAM), 96-well plate, Alprazolam, Benzodiazepines, Buprenorphine, Clonazepam, Codeine, Column, Diazepam, Drugs of Abuse, EDDP, English, Fentanyl, Flunitrazepam, Forensic, Hydrocodone, JWH 018-N-(4-hydroxypentyl), JWH-018, JWH-018, JWH-018-N-5-(pentanoic acid), JWH-073, JWH-073, JWH-073-N-(3-hydroxybutyl), JWH-200, JWH-250, JWH-250-N-(5-hydroxypentyl), LC-MS/MS, Morphine, Naltrexone, Nitrazepam, Normeperidine, Oral fluid , Oxazepam, Oxycodone, Oxymorphone, Posters, Synthetic Cannabinoids (SPICE), Temazepam, UR-144 , UR-144-(5-hydroxypentyl) , UR-144-(pentanoic acid) , XLR-11

Antiepileptic drugs are prescribed to suppress seizures in epilepsy patients. A variety of different types of AEDs have been synthesized to pharmacologically address different types of epilepsy. The ability to therapeutically monitor these drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of thedrug. A fast and clean extraction method is needed that works in a variety of biological matrices and affords a high throughput workflow. In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96 well plate format to facilitate a high throughput workflow model. MSACL 2014
Tags: 96-well plate, Antiepileptics, Carbamazepine 10,11 epoxide, Clinical, English, Felbamate, Gabapentin, LC-MS/MS, Oral fluid , Oxcarbazepine, Posters, Rufinamide, Saliva, Serum, Tigabatine, Urine, Vigabatrin

This poster demonstrates a simplified protocol for the simultaneous extraction of nicotine and its major metabolites including cotinine, nornicotine, 3-OH-cotinine, norcotinine and anabasine in plasma, urine, serum and whole blood using supported liquid extraction (SLE) followed by UPLC-MS/MS analysis. Pittcon 2014
Tags: 3-OH-cotinine, 96-well plate, Anabasine, Clinical, Cotinine, English, LC-MS/MS, Nicotine, Nicotine metabolites, Norcotinine, Nornicotine, Plasma, Posters, Serum, Urine

This poster demonstrates a simplified protocol for the simultaneous extraction of nicotine and its major metabolites including cotinine, nornicotine, 3-OH-cotinine, norcotinine and anabasine in plasma, urine, serum and whole blood using supported liquid extraction (SLE) followed by UPLC-MS/MS analysis. MSACL 2014
Tags: 3-OH-cotinine, 96-well plate, Anabasine, Clinical, Cotinine, English, LC-MS/MS, Nicotine, Nicotine metabolites, Norcotinine, Plasma, Posters, Serum, Urine, Whole blood

This poster demonstrates method development strategies for drugs of abuse screening using supported liquid extraction in a 96-well plate and subsequent method scalability to column format to allow larger matrix volume processing prior to UPLC-MS/MS analysis. MSACL 2014
Tags: Drugs of Abuse, English, Forensic, LC-MS/MS, Posters, Urine

This poster presents a simple method for the extraction and subsequent detection of both the traditional hydroxy metabolites and the biologically active dihydroxy metabolites in serum. High analyte recoveries and low ion suppression were demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites. MSACL 2014
Tags: 1, 25 Dihydroxyvitamin D, 1a, 25-dihydroxy-vitamin D, 1α, 25-dihydroxy-vitamin D, 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

Testing for drugs of abuse in oral fluids can strongly benefit the criminal justice field as a less invasive and cost-effective approach for drug detection when compared to blood or urine sampling. Oral fluid analysis has facilitated laboratory analysis for many drugs of abuse and is a constantly evolving analysis procedure which benefits from increasingly sensitive methods of detection. The Laser Diode Thermal Desorption (LDTD) source combined with Mass spectrometry is presented as a new screening tool for drug analysis in Oral Fluids. Analysis speed of LDTD provides accurate results in seconds in combination with exceptional specificity of MS instruments make a powerful platform for the screening of different drugs of abuse and new emerging drugs. Different extraction procedures are available; however those methods depend on specific drug conditions: basic or acid drugs / hydrophilic or hydrophobic. A new extraction approach, Supported Liquid Extraction (SLE+) is evaluated as generic extraction procedure. MSACL 2014
Tags: 96-well plate, Amphetamine, Benzoylecgonine, Butalbital, Cocaine, Codeine, Drugs of Abuse, English, Fentanyl, Forensic, LDTD-MS/MS, MDA, MDEA, MDMA, Methadone, Methamphetamine, Oral fluid , Phenobarbital, Posters, Secobarbital

This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme. MSACL EU 2014
Tags: 96-well plate, Clinical, Cortisol, English, LC-MS/MS, Posters, Serum

Free urinary cortisol levels can be an indicator of a variety of disorders, such as Cushing Syndrome. This poster presents a simple method for the extraction of cortisol from urine demonstrating good recoveries and low ion suppression. LC-MS/MS analysis was performed using a Waters 2795 Liquid Handling Unit coupled to a Quattro Ultima Pt triple quadrupole MS using electrospray ionization, operated in the MRM mode. Supported liquid extraction method development resulted in recoveries greater than 90% for cortisol spiked urine. Calibration curves constructed using this method from 25-2000 ng/mL, demonstrated excellent linearity with coefficients of determination greater than 0.99. Cortisol, Stress, Cushin's Syndrome, SLE, Supported Liquid Extraction, SPE, LLEMSACL, San Diego, 2013
Tags: Clinical, Cortisol, LC-MS/MS, Posters, Steroid Hormones, Urine

Immunosuppressant drugs are extremely important for therapeutic drug monitoring (TDM) regimes, requiring robust and reliable methods for their analysis. This poster aims to demonstrate strategies for the extraction of various immunosuppressant drugs from whole blood providing clean samples prior to UPLC-MS/MS analysis. Spiked whole blood was extracted using a variety of protocols to investigate optimal combination of drug recovery and extract cleanliness. UPLC-MS/MS was conducted using a Waters ACQUITY UPLC coupled to a Quattro Premier XE triple quadrupole mass spectrometer. Acceptable extraction recoveries were obtained showing excellent extract cleanliness, demonstrating a reliable method for mass spectrometric approaches for these analytes. Immunosuppressants, SLE, Supported Liquid Extraction, ISOLUTE, MSACL, San Diego, 2013
Tags: Clinical, Cyclosporin A, Everolimus, Immunosuppressant, LC-MS/MS, Posters, Sirolimus, Tacrolimus, Whole blood

Pain management therapy warrants constant monitoring of therapeutic levels of prescribed drug levels in patient urine samples. The number of samples being submitted for analysis has increased dramatically in the last 10 years with improvements in high throughput automated screening capabilities. Patient samples analysis is complicated by the need for an effective sample preparation methodology that can extract target analytes from complex matrices with good efficiency. Further complicating the process is the need to enzymatically hydrolyse the glucoronidated metabolites prior to extraction from the urine matrix. A fully automated sample preparation process using a TECAN Freedom EVO® 100 was designed to incorporate both the enzymatic hydrolysis and subsequent sample preparation assay as one continuous workflow. Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques was used to extract a suite of pain management drugs from spiked urine samples. A recovery and quantitation assay was run on the TECAN Freedom EVO® 100 using mock patient samples to demonstrate utility of automation process. MSACL, Pain Management, Biotage, SPE, SLE, LLE, Supported Liquid Extraction, Drugs, MSACL, San Diego, 2013
Tags: 6-acetylmorphine (MAM), 96-well plate, Benzodiazepines, Clinical, Clonazepam, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, Flunitrazepam, Hydrocodone, Hydrolysed urine, Hydromorphone, LC-MS/MS, Methadone, Methamphetamine, Morphine, Nitrazepam, Opiates, Oxycodone, Oxymorphone, Posters, Urine

The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in patient urine samples. Screening patient urine samples is further complicated by the metabolic process which converts the free drug to the - glucuronide form. The target drugs in patient urine samples can be enzymatically hydrolysed and extracted using Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques typically used for this type of clinical sample preparation. San Diego, MSACL, 2013
Tags: 6-acetylmorphine (MAM), Benzodiazepines, Clinical, Clonazepam, Codeine, Column, Diazepam, Drugs of Abuse, Flunitrazepam, Flurazepam, Hydrocodone, Hydrolysed urine, Hydromorphone, LC-MS/MS, Methadone, Methamphetamine, Morphine, Nitrazepam, Nordiazepam, Opiates, Oxazepam, Oxymorphone, Posters, Urine

Developed in collaboration with the OC Crime Lab, this poster describes the extraction of 25 benzodiazepines and sedatives from various matrices using ISOLUTE SLE+ Supported Liquid Extraction columns. This poster was presented at SOFT 2013.
Tags: 2-Hydroxyethylflurazepam, 7-Aminoclonazepam, 7-Aminoflunitrazepam, Alprazolam, Aqueous, Benzodiazepines, Brain, Bromazepam, Chlordiazepoxide, Clonazepam, Column, Demoxepam, Desalkylflurazepam, Diazepam, English, Estazolam, Flunitrazepam, Flurazepam, Forensic, Hydroxyalprazolam, Hydroxytriazolam, LC-MS/MS, Liver, Lorazepam, Midazolam, Nitrazepam, Nordiazepam, Oxazepam, Phenazepam, Pig's Blood, Posters, Stomach contents, Temazepam, Triazolam, Urine, Zaleplone, Zolpidem

The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread [5]. Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields. Cannabinoids, SLE, SLE+, Supported Liquid Extraction,
Tags: CP47,497, CP47,497-C8, Column, Forensic, HU210, JWH-018, JWH-073, JWH-200, JWH-250, JWH019, LC-MS/MS, Posters, Synthetic Cannabinoids (SPICE), Whole blood

The objective was to develop a GC-MS assay for the determination of free benzodiazepines using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods. ISOLUTE, SLE, SLE+, Supported Liquid Extraction, Benzodiazepines, Forensic, Drugs, DOA, Drugs of Abuse, SOFT,
Tags: 4-Hydroxynordiazepam, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 7-Aminonitrazepam, Benzodiazepines, Bromazepam, Column, Demethylflunitrazepam, Flurazepam, Forensic, GC-MS, Hydroxyalprazolam, Hydroxymidazolam, Hydroxytriazolam, Midazolam, Nitrazepam, Posters, Urine

Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. This poster presents a novel method for the extraction of 25 hydroxyvitamin D demonstrating acceptable validation parameters from serum calibrators and DEQAS supplied samples using ISOLUTE SLE+.
Tags: Posters

The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems
Tags: 96-well plate, Acidic, Biomarker, Column, English, LC-MS/MS, Methylmalonic acid (MMA), Posters, Serum

Steroid hormones are important physiological compounds that control inflammation, immune functions, salt and water balance, and development of sexual characteristics.
Tags: Posters

The objective was to develop a GC-MS assay for the determination of barbiturates from urine matrix using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods. Barbiturates, SLE, Supported Liquid Extraction, Forensic, SOFT, Urine,
Tags: Barbiturates, Butabarbital, Butalbarbital, GC-MS, Pentobarbital, Posters, Secobarbital, Urine

Endogenous phospholipids (outline structure shown in Figure 1.) present in biological fluids are a major problem in LC-MS/MS analysis. Due to their strong retention characteristics in reversed phase chromatography phospholipids tend not to elute as discrete peaks and are often very difficult to separate from analytes of interest. This co-elution often leads to areas of suppression or enhancement in the chromatogram which in turn can cause quantitation issues. Supported liquid extraction (SLE) is an analogous technique to traditional liquidliquid extraction. This poster compares phospholipid removal using a wide variety of solvent combinations, pH control and polar extraction solvents on supported liquid extraction plates ASMS 2011
Tags: English, Posters

Supported Liquid Extraction (SLE) is a 96-well high throughput technique that is analogous to traditional LLE.
Tags: Posters

Here we demonstrate a rapid and reliable 96-well Supported Liquid Extraction assay for the extraction of tamoxifen and metabolites from various human biological fluids.
Tags: Posters

It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating many of the tedious liquid handling issues associated with LLE.
Tags: Posters

It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating many of the tedious liquid handling issues associated with LLE.
Tags: Posters

Tags: Posters

Presented at ASMS 2007
Tags: Posters

This study suggests that supported liquid extraction using ISOLUTE®SLE+ 96-well extraction plates is a viable alternative to liquid-liquid extraction in a busy clinical laboratory.
Tags: Application Notes, Product Notes

Product note for ISOLUTE SLE+ 48-well plates
Tags: 48-well plate, English, Product Notes

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.
Tags: Product Notes

This Chemistry Data Sheet describes the use of ISOLUTE SLE+ Supported Liquid Extraction Plates for the isolation of acidic, basic and neutral drugs from biological fluids.
Tags: Application Notes, Product Notes

ISOLUTE® SLE+ Supported Liquid Extraction Plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, extracting up to 400 μL of aqueous sample. ISOLUTE SLE+ plates provide high analyte recoveries, eliminate emulsion formation, and cut sample preparation time in half.
Tags: Product Notes

This method card provides recommended conditions for using ISOLUTE® SLE+ Supported Liquid Extraction Plates and Columns.
Tags: Manuals & User Guides, Product Notes