This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Tags: 96-well plate, Acidic, Application Notes, Biomarker, Clinical, English, Extrahera, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Serum

ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation. This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin D, Vitamins

This application note describes the use of the ISOLUTE® PLD+ Protein and Phospholipid Removal Plate for clean-up of a range of acidic, basic and neutral drugs from plasma. ISOLUTE® PLD+ Protein and Phospholipid Removal Plates are suitable for clean-up of a range of analytes with widely differing functionality and polarity characteristics from plasma, giving high recoveries, good reproducibility and excellent extract cleanliness.
Tags: 96-well plate, Acetaminophen, Acidic, Amitriptyline, Application Notes, Atenolol, Basic, Bretylium, Brompheniramine, Clinical, English, Fluoxetine, Forensic, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, NSAIDs, Naltrexone, Neutral, Pharma, Plasma, Procainamide, Quinidine, Ranitidine, Salbutamol, Sulindac, Tricyclic Antidepressant, p-toluamide, β-blockers

In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs

This poster summarizes various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal. MSACL 2016
Tags: 96-well plate, Acidic, Clinical, English, LC-MS/MS, MMA (Methylmalonic acid), Methylmalonic acid (MMA), Posters, Serum

This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared. ASMS 2015
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Biomarker, Clinical, English, Extrahera, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego
Tags: 25-OH Vitamin D2, 25-OH Vitamin D3, 25-hydroxy-vitamin D, 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin D, Vitamins

This poster presents the ISOLUTE PLD+ Protein and Phospholipid removal plate, highlighting its ease of use and excellent performance with respect to sample clean up, analyte recovery and elimination of back pressure build up in the UPLC system. IMSC 2014
Tags: 96-well plate, Acetaminophen, Acidic, Amitriptyline, Atenolol, Basic, Bretylium, Brompheniramine, Clinical, English, Fluoxetine, Forensic, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Naltrexone, Neutral, Pharma, Pharmaceuticals, Plasma, Posters, Procainamide, Quinidine, Ranitidine, Salbutamol, Sulindac, p-toluamide

This poster evaluates the performance of a novel 96-well filter plate (ISOLUTE PLD+ Protein and Phospholipid Removal Plate) for the simultaneous removal of proteins and phospholipids from serum samples prior to LC-MS/MS analysis. ASMS2014
Tags: 96-well plate, Acidic, Amitriptyline, Analgesics, Atenolol, Basic, Bretylium, Clinical, English, Fluoxetine, Forensic, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, NSAIDs, Naltrexone, Pharma, Plasma, Posters, Procainamide, Quinidine, Ranitidine, Salbutamol, Serum, Sulindac, Tricyclic Antidepressant, β-blockers

The new ISOLUTE® PLD+ Protein and Phospholipid Removal Plate is highlighted in this poster. Protein and phospholipid removal, and analyte recovery are included, along with data illustrating the positive impact of clean (PL and protein free) samples in maintaining analyte signal intensity and low UPLC column back pressure over multiple LCMS runs. HPLC 2014
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters

User instructions:ISOLUTE® PLD+ Protein and Phospholipid Depletion Columns
Tags: Clinical, Column, English, Extrahera, Forensic, LC-MS/MS, Pharma, Product Notes

User Instructions: ISOLUTE® PLD+ Protein and Phospholipid Depletion Plates
Tags: 96-well plate, Clinical, English, Extrahera, Forensic, Pharma, Product Notes

FAQs: ISOLUTE® PLD+ Protein and Phospholipid Removal Products
Tags: 96-well plate, Clinical, Column, English, Forensic, LC-MS/MS, Pharma, Product Notes