This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Tags: Acenaphthene, Acenapthalene, Aliphatic hydrocarbons, Anthracene, Application Notes, Aromatic hydrocarbons, Benzo(a)anthracene, Benzo(a)pyrene, Benzo(b)fluoranthene, Benzo(ghi)perylene, Benzo(k)Fluoranthene, Chrysene, Column, Dibenzo(ah)anthracene, English, Environmental, Extrahera, Fluoranthene, Fluorene, GC-MS, Indeno(123-cd)pyrene, Napthalene, Phenanthrene, Polyaromatic Hydrocarbons (PAH), Pyrene, Soil
This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system.
EPH, Massachusetts Department of Environmental Protection,
Tags: Aliphatic hydrocarbos, Application Notes, Column, Environmental, Environmental contaminants, Polyaromatic hydrocarbons, RapidTrace, Soil
ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction.
Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,
Tags: Aliphatic hydrocarbos, Application Notes, Column, English, Environmental, Polyaromatic hydrocarbons, Soil
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
This Chemistry Data Sheet provides procedures for both the automated and manual fractionation of EPH in soil. The
automated procedure is described on page 2 and the manual procedure is outlined on page 3. Chromatograms
illustrate the ability of ISOLUTE EPH to provide efficient fractionation of hexane or pentane soil extracts into
aliphatic and aromatic fractions (see Appendix).
Tags: Product Notes, Soil
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What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >