Sample Preparation
Horizon Food & Environmental Products
Organic Synthesis
Peptide Chemistry
Flash Purification
Work-up Products
Evaporation & Concentration
Process Development
Industrial Products
Service & Support
- Automated Sample Preparation Instruments
- Manual Sample Preparation & Accessories
- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
- ISOLUTE® SLE+ Supported Liquid Extraction Products
- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
- ISOLUTE® C18(EC)
- ISOLUTE® C2
- ISOLUTE® C2(EC)
- ISOLUTE® C4
- ISOLUTE® C8
- ISOLUTE® C8(EC)
- ISOLUTE® CBA
- ISOLUTE® CN
- ISOLUTE® DIOL
- ISOLUTE® ENV+
- ISOLUTE® ENV+ On-line SPE Cartridges
- ISOLUTE® EPH
- ISOLUTE® FL
- ISOLUTE® HAX
- ISOLUTE® HCX
- ISOLUTE® HCX-3
- ISOLUTE® HCX-5
- ISOLUTE® HCX-Q
- ISOLUTE® HM-N Columns
- ISOLUTE® Layered SPE Columns
- ISOLUTE® MFC18
- ISOLUTE® Multimode
- ISOLUTE® Myco
- ISOLUTE® NH2
- ISOLUTE® Na2SO4/FL
- ISOLUTE® O&G
- ISOLUTE® PAH
- ISOLUTE® PE-AX
- ISOLUTE® PH
- ISOLUTE® PSA
- ISOLUTE® SAX
- ISOLUTE® SAX/PSA
- ISOLUTE® SCX
- ISOLUTE® SCX-2
- ISOLUTE® SCX-3
- ISOLUTE® SI
- ISOLUTE® Sodium Sulfate Drying Cartridge
- ISOLUTE® TPH
- EVOLUTE® SPE Columns and Plates
- AFFINILUTE™ MIP Columns
- Service Agreements - Europe
- Service Agreements - North and Latin America
- Discontinued Products
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- Biotage® Syro Wave™
- Biotage® Advancer
- Biotage® Atlantis
- Biotage® ELSD-1080
- Biotage® Initiator Classic
- Biotage® Resolux™
- Biotage® SP
- Biotage® V-10 Evaporator
- EVOLUTE® ABN
- EVOLUTE® AX
- EVOLUTE® CX
- EVOLUTE® WAX
- EVOLUTE® WCX
- FlashMaster Personal Plus
- KILOPREP Systems and Consumables
- SAM
- SAP
- TurboVap® Classic 500
- TurboVap® Classic II
- TurboVap® Classic LV
C40708
Test Tubes (16 x 100 mm, Uncap)
Read more on the product page
RapidTrace®+ » TurboVap® Classic LV » Biotage® Extrahera™ » TurboVap® LV »
» APPLICATION NOTES
Automated Solid Phase Extraction for Cyclosporine in Blood
Cyclosporine is an immunosuppressant used in organ transplants to overcome rejection. Following the transplant of a heart, liver, kidney or lung, a patient must be regularly tested for the rest of their lives. Blood testing intervals vary from 1 month to 6 months and this rapid reference method is ideal for batch tests with the advantage of no metabolite interfer-ence which is always found in immunological methods.
Tags:
Application Notes,
Liver
Automated Solid Phase Extraction for Environmental Samples V.1
The removal of interferences and sample concentration are
essential steps in trace level detection of pesticides, sheep
dips, herbicides and PCBs in river water, wastewaters and
trade effluents. The following methods were developed by
the Environment Agency National Laboratory Service facility
at Nottingham using the Biotage RapidTrace for automated
solid phase extraction and the TurboVap II for evaporation.
The procedures are UKAS accredited and can reach detection
limits of 0.001 ug/Litre for some compounds.
Tags:
Application Notes,
Environmental,
Environmental contaminants,
Organophosphates,
RapidTrace,
Water
Automated Solid Phase Extraction of Tricyclic Antidepressant Drugs from Serum
With the widespread clinical use of antidepressant drugs comes the need to accurately measure therapeutic lev-els to determine proper dosage, as well as check for toxic levels and patient compliance. More and more laboratories are shifting their therapeutic drug monitor-ing from liquid extraction to solid phase extraction. A large clinical laboratory has automated the process of extracting these drugs from serum using the RapidTrace SPE Workstation.
Tags:
Application Notes
Automated Solid Phase Extraction of Dioxins and Furans in Soil and Sediments V.1
EPA methods 8260 & 8270 cover the analysis of polychlorinated
dibenzo-p-dioxins and polychlorinated dibenzofurans
in ground & surface waters. This also now includes
1,4-dioxane and PCBs. This application note will focus on
the automated SPE of the sample rather than the detection
which usually involves GCMS or LCMS. NB The columns
and reagents used for soils and sediment analysis differ to
ground water extraction.
Tags:
Application Notes,
Environmental,
Environmental contaminants,
RapidTrace,
Soil
Extraction of Pain Management Drugs from Urine using EVOLUTE® CX With the RapidTrace+ Automated SPE Workstation Prior to LC-MS-MS
The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed to cleave glucuronide moiety and produce the free form of drug. The target analytes can them be extracted from the urine matrix using EVOLUTE CX solid phase extraction cartridges. LOQs range from 5 ng/mL to 0.5 ng/mL with recoveries above 80% and RSD <10%
Pain Management, EVOLUTE, CX, SPE, Solid Phase Extraction, Polymer, LC-MS-MS, Cocaine, Diazepam, Heroin, Morphine, Oxycodone, Fentanyl, Diazepam, Mixed Mode, RapidTrace
Tags:
Alprazolam,
Application Notes,
Buprenorphine,
Butylone,
Clinical,
Clonazepam,
Codeine,
Column,
Fentanyl,
Flunitrazepam,
Hydrocodone,
Hydromorphone,
LC-MS/MS,
Meperidine,
Methadone,
Morphine,
Naloxone,
Naltrexone,
Oxycodone,
Oxymorphone,
RapidTrace,
Temazepam,
Urine
Extraction of Melamine from Milk (Infant Formula) using EVOLUTE® CX Columns and RapidTrace+ Workstation
Melamine is traditionally used in making plastics, however, it’s low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows recoveries of 88-90% with RSDs <10%.
Melamine, food scare, china, EVOLUTE, cation exchange, RapidTrace, Automation, SPE, sample prep,
Tags:
Application Notes,
Column,
Food,
Food contaminant,
LC-MS/MS,
Melamine,
Milk,
RapidTrace
Extraction of Ethyl Glucuronide (EtG) from urine using EVOLUTE® AX and RapidTrace+ Workstation
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Tags:
Alcohol metabolite,
Application Notes,
Column,
Ethyl glucuronide (EtG),
LC-MS/MS,
RapidTrace,
Urine
Method for the extraction of Morphine and Codeine from urine using ISOLUTE® HCX and RapidTrace® Workstation
This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation.
Opiates, drug testing, DOA, Ion exchange
Tags:
Application Notes,
Codeine,
Column,
Forensic,
Morphine,
Opiates,
RapidTrace,
Urine
Method for the Extraction of Cyclosporine from Whole Blood Using ISOLUTE® SI Columns and RapidTrace® Workstation
Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid.
Cyclosporine, Cyclosporine, Immunosuppressant,
Tags:
Application Notes,
Clinical,
Column,
Cyclosporin,
Cyclosporine,
Immunosuppressant,
RapidTrace,
Whole blood
Method for the Extraction of Basic Drugs of Abuse from Biological Fluids using ISOLUTE® HCX & RapidTrace® Workstation
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Tags:
Amitriptyline,
Amphetamine,
Application Notes,
Benzoylecgonine,
Biological fluids,
Butylone,
Codeine,
Column,
Desipramine,
Forensic,
Imipramine,
Methamphetamine,
Morphine,
Nortryptiline,
Plasma,
RapidTrace,
Tricyclic Antidepressant,
Urine
Method for Extraction of Tricyclic Antidepressants from Whole Blood Using ISOLUTE® HCX Columns and RapidTrace®
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Tags:
Amitriptyline,
Application Notes,
Clinical,
Column,
Desipramine,
Imipramine,
Nortryptiline,
RapidTrace,
Tricyclic Antidepressant,
Whole blood
Method for the Extraction of the Cocaine Metabolite, Benzoylecgonine from Urine using ISOLUTE® HCX and RapidTrace®
This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.
Tags:
Application Notes,
Benzoylecgonine,
Cocaines,
Column,
Forensic,
RapidTrace,
Urine
RapidTrace Procedure for the Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil using ISOLUTE EPH SPE Columns
This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system.
EPH, Massachusetts Department of Environmental Protection,
Tags:
Aliphatic hydrocarbos,
Application Notes,
Column,
Environmental,
Environmental contaminants,
Polyaromatic hydrocarbons,
RapidTrace,
Soil
Automated SPE Extraction of Benzoylecgnonine (BZE) from Urine for Confirmation by GC/MS
When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.
Tags:
Application Notes,
Benzoylecgonine,
Column,
Drugs of Abuse,
Forensic,
GC-MS,
RapidTrace,
Urine
Automated Solid Phase Extraction of Organic Compounds from Marine Sediments, Soil, and Dust Samples
The extraction and segregation of Organic compounds from core samples taken from marine sediments is used to predict climate conditions, reconstruct sea temperatures and identify the type of vegetation from the C3 ( Temperate cli-mate) and C4 ( Tropical Climate) isotopic mass spectroscopy ratio analysis.
Tags:
Application Notes
SPE Application Note for Extraction of NIDA-5 (SAMHSA-5) Analytes using RapidTrace
This method details extraction of the NIDA-5 (SAMHSA-) drug of abuse classes using the same SPE column (ISOLUTE HCX 130 mg/1 mL) and single set of reagents (8 in total) on the RapidTrace.
Tags:
Application Notes,
RapidTrace
SPE Application Note for 6-monoacetyl Morphine from Urine using RapidTrace
The following method addresses the extraction of 6-monoacetyl morphine from urine using
non-polar and strong cation exchange retention mechanisms. This compound can confirm
heroine use since it is a heroine metabolite, but not a metabolite of codeine or morphine
Tags:
Application Notes,
Codeine,
RapidTrace,
Urine
» BROCHURES
RapidTrace® + Automated SPE Workstation
The RapidTrace+ is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated
pharmaceutical, clinical and forensic laboratories. The RapidTrace+ eliminates SPE bottlenecks so you can realize the
full benefits of today’s powerful analytical instruments.
Tags:
Brochures
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags:
Catalogs
» CERTIFICATES
Declaration of Conformtiy RapidTrace January 2018
Declaration of Conformtiy RapidTrace
Tags:
Certificates,
Clinical,
English,
Environmental,
Food,
Forensic,
Product Notes
» CUSTOMER CASES
RapidTrace Automated Solid-Phase Extraction (SPE) System: Best Performer in Massive Requirements for the Doping Tests of Racing Horses
User report: RapidTrace. The Laboratory of Racing Chemistry (LRC) analyzes samples from racehorses for doping drug. In order to screen approximately 1000 samples per week, LRC use an array of Biotage RapidTrace automated SPE systems. In an interview, Mr. Kenji Kinoshita and Mr. Kensaku Shirai talked about their experiences with the RapidTrace system in processing the heavy workload.
Tags:
Customer Cases
» POSTERS
Application of a GC-MSMS Method for the Simultaneous Determination of Persistent Organic Pollutants in Serum from California First Time Mothers (PN73)
A GC-MSMS method of simultaneously measuring legacy/emerging POPs
PCBs, OCPs and PBDEs) in serum was developed and applied to this study of exposure of mothers to harmful environmental contaminants.
Tags:
BHC,
Clinical,
DDE,
DDT,
English,
Environmental,
GC-MS,
HCB,
Organochlorine pesticides (OCP),
Organochlorines,
Oxychlordane,
PBDE,
PBDEs,
PCB,
PCBs,
Persistant organic pollutants (POP),
Posters,
RapidTrace,
Serum,
Trans-nonachlor
An automated dual stage solid phase extraction procedure for 15 F2t-isoprostane (8-iso Prostaglandin2a) from BSA as lipid markers of oxidative stress
This poster describes an automated procedure for isolation and clean up of 15 F2t-isoprostane (8-iso Prostaglandin 2a)(a lipid marker of oxidative stress) from BSA.
Tags:
15 F2t-isoprostane,
8-iso Prostaglandin2a,
BSA,
Biological fluids,
Biomarker,
Clinical,
Column,
English,
GC-MS,
Lipids,
Posters,
RapidTrace
» PRODUCT NOTES
Declaration of Conformtiy RapidTrace January 2018
Declaration of Conformtiy RapidTrace
Tags:
Certificates,
Clinical,
English,
Environmental,
Food,
Forensic,
Product Notes
Cleaning and Maintenance of RapidTrace® Workstation
In order to keep your RapidTrace® workstation clean, in good working order and free from contamination it is of course essential to
routinely clean and purge the fluid path. Whichever matrix and reagents are running through the system, traces of these liquids will
be left after use; proteins from plasma, precipitates from buffers or even particulates from oral fluid. Over time if the following
cleaning schedule is not observed these contaminants could cause blockages leading to malfunctions and/or cross contamination.
Tags:
Product Notes
Search all documents »
INSTRUCTION VIDEOS
Biotage RapidTrace®+ SPE Workstation
News
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
20 April 2017Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
18 February 2015The Biotage Innovation Award Goes to...
Every year, Biotage awards an employee that has come up with the best idea resulting in a successful new product or product improvement that makes it possible for Biotage to offer their custom...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
01 October 2014New Automated Sample Preparation System
Uppsala, Sweden, 1st October 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of the new...
13 January 2014Easier Cyclosporine Monitoring After Organ Transplant
Following the transplant of a heart, liver, kidney or lung, patients must be regularly tested for cyclosporine during the rest of their lives. Cyclosporine is an immunosuppressant us...
18 October 2011Biotage Introduces Automated SPE Workstation for Increasing Productivity in Sample Preparation
October 18, 2011 Uppsala, Sweden – Biotage® (STO: BIOT), a leading manufacturer of tools and technology for analytical and medicinal chemistry, introduced the new RapidTrace®+ SPE Wor...
26 March 2011Biotage Releases Comprehensive New Catalog of Sample Preparation Products and Applications
Biotage announced the release of its new Analytical Sample Preparation Catalog. The expanded, 200-page catalog includes a range of products for Bioanalytical, Forensic, Clinical, Environmental, Agr...
10 January 2011Biotage Announces New Methods for the Extraction of Key Compounds for Clinical, Environmental and Forensic Applications
Charlotte, North Carolina USA, January 7, 2011 Biotage® (STO: BIOT), a leading supplier of tools and technology for analytical and medicinal chemistry, introduced a new series of application no...
Q&A
Max sample volume on RapidTrace+
31 July 2018Question:
My SPE technique is RapidTrace+. Is it possible to apply bigger sample volume (1000 mL) without prior evaporation?
Answer:
The maximum sample volume in standard operational mode is 40 mL limited to a 5.8 mL load each time. This is limited by the sample vial size and will only give you a 5 sample capacity.
Loading 40 mL of sample will be achieved by 6 x 5.8 mL then an additional 5.2 mL.
If you were to use one of the reagent lines as a source and use the condition function then it is possible to load more sample contained in an external vessel onto the SPE column via the reagent line. You will only be able to process a limited number of samples as dictated by other parameters within your SPE method as they will use reagent lines. Additionally you will not be able to load 1000 mL as you are limited by programming lines in a particular extraction procedure.
Essentially the RapidTrace is not really the right tool for the job. You would be better off considering the Biotage® Horizon 5000 depending on your application http://www.biotage.com/product-page/biotage-horizon-5000
Browse more questions and answers >INSTRUMENTS
| Part No. | Description | Product type | Pack Size | Price | ||
|
C125713 | RapidTrace®+ 6 mL | Instruments | 1 | Log in for price | |
|
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C50000 | RapidTrace®+ 3 mL | Instruments | 1 | Log in for price | |
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
C40707 | Test Tubes (13 x 100 mm, Uncap) | Consumables | 1000 | Log in for price | |
|
|
C40708 | Test Tubes (16 x 100 mm, Uncap) | Consumables | 1000 | Log in for price | |
