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Biotage® ACT Plate Adapter
Protect Your Samples from Cross Contamination During Evaporation
Biotage® ACT (Anti Cross Talk) Plate Adapter is a novel (patent pending) solution to the phenomenon of hot spot cross contamination, or cross talk, during evaporation.
» BROCHURES
Avoiding Cross Talk In 96-well Based Sample Preparation
This guide describes simple practical strategies, hints and tips designed to mitigate or avoid cross-well contamination or ‘cross talk’ in high throughput 96-well based sample preparation techniques.
Tags: 96-well plate,
Brochures,
Clinical,
English,
Forensic,
Pharma
» MANUALS & USER GUIDES
Care and User Instructions Biotage® ACT Plate Adapter
Biotage® ACT Plate Adapter (patent pending) is designed to reduce or eliminate the occurrence of well-to-well cross contamination (cross talk) during extract evaporation, and fits on top of the 96-well collection plate during evaporation.
Tags: Manuals & User Guides
» POSTERS
Evaluation of Novel Automated Sample Preparation Compared to Manual Processing in Forensic Toxicology
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination.
TIAFT 2016
SOFT 2016
Tags: 2-Hydroxyethylflurazepam,
2-OH-Et-Flurazepam,
6-acetylmorphine (MAM),
7-Aminoclonazepam,
7-Aminoflunitrazepam,
96-well plate,
Alprazolam,
Amphetamine,
Amphetamines,
Benzodiazepines,
Bromazepam,
Buprenorphine,
Clinical,
Clonazepam,
Codeine,
Column,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Estazolam,
Extrahera,
Fentanyl,
Flunitrazepam,
Flurazepam,
Forensic,
GC-MS,
Hydrocodone,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDA,
MDEA,
MDMA,
Mephedrone,
Methadone,
Methamphetamine,
Midazolam,
Morphine,
Nitrazepam,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norketamine,
Opiates,
Opiates,
Oxazepam,
Oxycodone,
Oxymorphone,
Phencyclidine (PCP),
Posters,
Temazepam,
Triazolam,
Urine,
Whole blood,
Zaleplone,
Zolpidem,
Zopiclone,
z-drugs,
α-OH Triazolam,
α-OH alprazolam
Extraction of Urinary Hormone Metabolites using Supported Liquid Extraction prior to HPLC-MS/MS Analysis
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
MSACL 2016
Tags: 11-deoxycortisol,
11-keto-androsterone,
11-oxoetiocholanolone,
11-β-hydroxyandrosterone,
11β-hydroxyetiocholanolone,
2-OH-estrone,
2-methoxyestrone,
3α-androstanediol,
3β-androstanediol,
4-OH-estrone,
4-methoxyestrone,
96-well plate,
Allo-THB,
Androsterone,
Clinical,
Corticosterone,
DHEA (dehydroepiandrosterone,
Dehydroepiandrosterone (DHEA),
English,
Etiocholanolone,
Extrahera,
Hydrolysed urine,
Posters,
Steroid Hormones,
Steroid metabolite,
THB,
THF,
Testosterone,
α-THF
Exploring the Sources of Cross Contamination in 96-Well Sample Preparation Prior to LC-MS/MS Analysis
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
MSACL 2016
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
Evaluating the Potential for Cross Contamination when performing 96-well Sample Preparation prior to LC-MS/MS Analysis
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Opiates,
Pharma,
Posters
» PRODUCT NOTES
Biotage® SPE Dry 96 Sample Concentrator System
Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing,
SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Tags: English,
Product Notes
Biotage® ACT Plate Adapter
This product note details technical and ordering information for the Biotage® ACT Plate Adapter, a novel accessory for prevention of cross talk during evaporation.
Tags: 96-well plate,
Clinical,
English,
Forensic,
Pharma,
Product Notes
Search all documents »
News
27 September 2016Automated vs. Manual Sample Preparation in Forensic Toxicology
Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices. Data reliability in forens...
16 July 2015Press Release – Biotage® ACT Plate Adapter Prevents Evaporative Cross-Talk
Uppsala, Sweden, 15th July 2015 – Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, announces the launch of Biotage® ...
Easy to incorporate into any 96-well based assay, this essential evaporation accessory can save the cost and time of sample re-runs.
The simple but effective design prevents cross talk on evaporation, decreasing the risks of falsely elevated results. Its high specification anodized aluminum construction allows it to be reused, and it is easily cleaned between runs to prevent any potential carry over.
Biotage ACT Plate Adapter is designed for use with square well colection plates, and is compatible with 'straight needle' 96-well plate evaporators such as the Biotage SPE Dry 96 and SPE Dry 96 Dual Sample Concentrator Systems.
Download the Product Note
Find out more about potential causes of cross talk in 96-well sample preparation, and how to avoid them, in our comprehensive guide
Find out more about LC-MS/MS troubleshooting, and approaches to solving the problem of false positives in the context of a high throughput clinical laboratory in this presentation (scroll to 'LC-MS/MS Troubleshooting at a Large Reference Lab' by Alan Rockwood, PhD, courtesy of MSACL.org)
ACCESSORIES
| Part No. | Description | Product type | Pack Size | Price | ||
|
|
414355SP | Biotage® ACT Plate Adapter | Accessories | 1 | Log in for price | |
The Problem
There are many potential causes of sample cross contamination in any clinical or forensic toxicology assay, particularly with the increasing use of the high throughput, low volume 96-well pate format for sample preparation. One recently identified area of concern is during evaporation.
During the evaporation stage, a proportional amount of analytes present in an extracted sample can contaminate surrounding wells by being carried with the volatilized solvent. When analyte concentration in the “hotspot” well is considerably higher than the analyte concentration in the surrounding wells the potential exists for extraneous analyte signal in adjacent and/or surrounding wells, thus leading to false positives which could pose severe consequences in any assay.
Mechanism for ‘hot spot’ cross talk during evaporation, resulting in carry-over from high-concentration sample wells to low-concentration wells, which show up as false positives.
The Solution
Biotage® ACT Plate Adapter fits on top of the 96-well collection plate during evaporation. Evaporation gas and solvent vapours are directed away from sample tubes, eddies are reduced, and the adapter reduces the open surface area of the top of the wells, lessening the likelihood that material can re-enter and contaminate adjacent wells.
