This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

The DryDisk® Separation Membrane was designed to replace conventional sodium sulfate in the drying of sample extracts. The DryDisk® is a hydrophobic membrane, consisting of a GORETEX ® process filtration media based on polytetrafluroethylene (PTFE). The hydrophobic property of the membrane allows the extracting solvent to pass through it, while retaining any water in the sample extract on the membrane’s surface.

Application Notes English Environmental Organic Compounds Water

Effective removal of residual water from organic solvents is critical to achieve optimal recoveries for water soluble compounds. Sodium sulfate has been the standard technique for removing residual water from solvent extracts for many years; however, it has several major drawbacks. Water soluble analytes in the residual water phase can become adsorbed in the sodium sulfate, leading to lower recoveries.

Application Notes Disk English Environmental GC-MS Organic Compounds Water

This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.

2,4,5-T 2,4-D 2,4-DB Acid herbicides Application Notes Asulam Benazolin Bentazone Bromoxynil Dicamba Dichlorprop Drinking water English Environmental Ioxynil MCPA MCPB Mecoprop On-line SPE On-line SPE-LC-MS/MS Pentachlorophenol Quinmerac Surface water Triclopyr Water

Pesticides, including insecticides, herbicides and fungicides are used extensively to increase agricultural yields. The total world usage of pesticides in 2007 was approximately 5.2 billion pounds with the US consuming approximately 22% of the total.1 The wide use of pesticides yields concern that drinking water sources will become contaminated, exposing the population to hazardous substances that may cause cancer.

Application Notes Disk Drinking water EPA 508.1 Analytes English Environmental GC-MS Pesticides

US EPA Method 1664 for Hexane Extractable Material (HEM) or Oil and Grease has allowed use of solid phase extraction (SPE) instead of liquid-liquid extraction (LLE) with hexane since 2007 and this has been widely adopted in the US. SPE is an equivalent extraction technique to LLE and produces the same n-hexane extract. The extract, similar to LLE, may contain residual water that must be treated properly and removed from the n-hexane extract.

Environmental Posters

This application note demonstrates that the Biotage(R) Horizon 4790 using Atlantic C18 disks can effectively extract hormone compounds from 1-L water samples in approx 40 mins.

11-ketotestosterone 17-alpha-estradiol 17-alpha-ethynylestradiol 17-beta-estradiol 4790 Androgens Androsterone Application Notes Diethylstilbestrol Dihydrotestosterone (DHT) Disk Endocrine disrupter English Environmental Epitestosterone Estradiol Estriol Estrogens Estrone GC-MS Hormones Mestranol Norethindrone Progesterone Steroid Hormones Steroid metabolite Surface water Testosterone Water

Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.

Application Notes Column Drinking water English Environmental LC-MS/MS Polyfluorinated acids

This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water, wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.

4790 Acetaminophen Albuterol Albuterol Application Notes Atenolol Caffeine Carbamazepine Cotinine DEET Dehydronifedipine Diclofenac Diltiazem Diphenhydramine hydrochloride Disk Drinking water English Environmental Gemfibrozil Ground water Ibuprofen LC-MS TOF Lamotrigine Metoprolol Naproxen Pharmaceuticals Sulfadimethoxine Sulfamethoxazole Triclocarban Trimethoprim Waste water

This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.

Application Notes Atenolol Atorvastatin Azithomycin Carbamazepine Ciprofloxacin Clarithromycin Diclofenac English Environmental Erythromycin Fluoxetine On-line SPE On-line SPE-LC-MS/MS Pharmaceuticals Prednisolone Ranitidine Sertraline Tolytriazole Water

Pesticides have gone far in increasing food supply, important in a world expecting to have 9.8 billion people by 2050. However, the mechanisms that make pesticides effective in eliminating pests can also cause harm to humans and animals. News stories continue to point to contamination through spills and exposure through the respiratory system.

5000 Application Notes Disk EPA 608.3 Analytes English Environmental GC-MS US EPA Method 608.3 Waste water

A GC-MSMS method of simultaneously measuring legacy/emerging POPs PCBs, OCPs and PBDEs) in serum was developed and applied to this study of exposure of mothers to harmful environmental contaminants.

BHC Clinical DDE DDT English Environmental GC-MS HCB Organochlorine pesticides (OCP) Organochlorines Oxychlordane PBDE PBDEs PCB PCBs Persistant organic pollutants (POP) Posters RapidTrace Serum Trans-nonachlor

Polychlorinated Biphenyls (PCB) are a worldwide contamination problem. They are extremely stable and as such, very long lived in the environment. The end result is that PCBs tend to bioaccumulate throughout the food chain and a great deal of research and regulations have been dedicated to their study.

4790 Application Notes English Environmental GC-MS PCB PCBs US EPA Method 8082 Water

ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction. Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,

Aliphatic hydrocarbos Application Notes Column English Environmental Polyaromatic hydrocarbons Soil

In this application note, a suite of neutral and list 1 pesticides in methylene chloride is evaporated using the Biotage® DryVap. Pesticides containing many different functional groups, including sulfur and phosphorus groups, halides, nitramines, atrazines, alcohol, and carbonyl groups were efficiently concentrated for GCMS analysis. This data indicates that the DryVap System is inert and effectively manages solvent evaporation without generating localized hot spots in the system.

Application Notes English Environmental GC-MS Organochlorine pesticides (OCP) Organophosphate Pesticide Pesticides Water

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.

4790 Anthracene Application Notes Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Disk EN16691 Analytes EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Surface water

This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs. Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.

4790 Anthracene Application Notes Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo(b)fluoranthene Benzo(k)Fluoranthene Disk EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Water

This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.

1,2 4 trichlorobenzene 1,2,3 trichlorobenzene 1,3,5 trichlorobenzene 5000 Alachlor Aldrin Application Notes DDD DDE DDT Dieldrin Disk Drinking water EN16693 Water Quality EN16693:2015 Analytes Endosulfan Endrin English Environmental GC-MS Hexachlorobenzene Hexachlorobutadiene Isodrin Organochlorine pesticides (OCP) Pentachlorobenzene Water alpha HCH beta HCH delta HCH gamma HCH

The removal of interferences and sample concentration are essential steps in trace level detection of pesticides, sheep dips, herbicides and PCBs in river water, wastewaters and trade effluents. The following methods were developed by the Environment Agency National Laboratory Service facility at Nottingham using the Biotage RapidTrace for automated solid phase extraction and the TurboVap II for evaporation. The procedures are UKAS accredited and can reach detection limits of 0.001 ug/Litre for some compounds.

Application Notes Environmental Environmental contaminants Organophosphates RapidTrace Water