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Methods

Chinese Method SL-392-2007 EN1664 Water Quality EN16691 Water Quality EN16693 Water Quality US EPA Method 1664 US EPA Method 3535A US EPA Method 506 US EPA Method 508.1 US EPA Method 515.2 US EPA Method 521 US EPA Method 525.2 US EPA Method 525.3 US EPA Method 526 US EPA Method 535 US EPA Method 537 US EPA Method 548.1 US EPA Method 549.2 US EPA Method 552.1 US EPA Method 608 US EPA Method 608.3 US EPA Method 625 US EPA Method 8015 US EPA Method 8061 US EPA Method 8081 US EPA Method 8082 US EPA Method 8141 US EPA Method 8270D US EPA Method 8330B

Format

4790 48-well plate 5000 96-well plate Bead Ruptor Column Disk Extrahera Lysera On-line SPE QuEChERs RapidTrace SmartPrep TECAN

Analytical Technique

GC-FID GC-FPD GC-MS GC-PID GC/ECD GC/MS/MS Gravimetric HPLC HPLC-DAD LC-MS TOF LC-MS/MS LDTD-MS/MS On-line SPE-LC-MS/MS UPLC-MS/MS

Analyte Type

Acid herbicides Acidic Acidic catecholamine metabolites Acifluorfen Adipate esters Alcohol metabolite Aliphatic hydrocarbons Alkaloids Aminoglycoside antibiotics Amphetamines Anabolic steroids Anaesthetic Analgesics Androgens Antibiotics Anticoagulant Anticonvulsant Antiepileptics Antimicrobial Antipsychotics Aromatic hydrocarbons B Vitamins Barbiturates Base-neutral herbicides Basic Benzodiazepines Beta blockers Biomarker Brominated Flame Retardants (BFRs) Bronchodilator Cannabinoid Carbamate hypnotic Carbamate muscle relaxant Carbamates Catecholamines Cathinones Chinese Environmental Agency Method Chlorinated acids Cocaines Corrosion inhibitors Corticosteroids Designer stimulants Diarrhetic Shellfish Poisoning (DSP) Toxins Diesel Range Organics Dinoseb Dioxins Diuretics Dried chili Drugs of Abuse EN16691 Analytes EN16693:2015 Analytes EPA 1657 Analytes EPA 1664 A Analytes EPA 1664 B Analytes EPA 1668A Analytes EPA 506 Analytes EPA 508.1 Analytes EPA 521 Analytes EPA 522 Analytes EPA 525.2 Analytes EPA 525.3 Analytes EPA 535 Analytes EPA 548.1 Analytes EPA 549.2 Analytes EPA 550.1 Analytes EPA 608 Analytes EPA 608.3 Analytes EPA 625.1 Analytes EPA 8015 Analytes EPA 8061A Analytes EPA 8081 Analytes EPA 8270 Analytes EPA 8330B Analytes Endocrine disrupter Environmental contaminants Estrogens Explosives Fat Soluble Vitamins Fentanyls Fluoroquinolones Food Additives Food Colours Food contaminant Hallucinogen Haloacetic Acids Hexabromobiphenyl Hexane Extractable Material High Boiling Solvents Hormones Hydrocodone Imidazoline corrosion inhibitor Imidazolines Immunosuppressant Insecticides Ketamine Legal highs Lipids Marine Toxins Metanephrines Micocystins Mycotoxin NBOMes NSAIDs Neutral Nicotine metabolites Nitroimidazoles Nitrosamines Oil & Grease Opiates Opioid Opioid agonist Organic Compounds Organochlorine pesticides (OCP) Organophosphate Pesticide Over the counter pharmaceuticals PAHs PBDEs PCBs PFAA PFAAs PPCPs Pain Management Paralytic Shellfish Poisoning (PSP) Toxins Peptides Persistant organic pollutants (POP) Personal Care Products Perylene-d12 Pesticides Pharmaceuticals Phenethylamines Phenolic Compounds Phenols Phthalate Metabolites Phthalates Phthalic acid esters Plasticisers Polyaromatic Hydrocarbons (PAH) Polyfluorinated acids Preservative Quaternary Amines RDX SERM (selective estrogen receptor modulator) SL-392-2007 SVOC Semi-Volatile Organics Solvent Exchange Spice Steroid Hormones Steroid metabolite Stimulants Sympathomimetic amine Synthetic Cannabinoids (SPICE) THC metabolites Thyroid Hormone Tobacco Specific Nitrosamines (TSNAs) Triazine Herbicides Triazines Tricyclic Antidepressant Triphenylmethane Dyes Veterinary drugs Vitamin D Vitamins Water Soluble Vitamins tetryl z-drugs β-agonists β-blockers

Matrix

Animal feed Apple juice (clear) Aqueous BSA Barley Biological fluids Bovine kidney Brain Brazil nut Breast milk Carbonated soft drink (soda) Chicken meat Coffee Dried chili Drinking water Eggs Enzyme powder Fish Food Grain Ground water Groundnut (Peanut) Hair Hemolysed whole blood Honey Hydrolysed urine Infant food (cereal) Infant formula (milk) Liver Maize Meat Meconium Milk Mouse plasma Nail (human) Nuts Olive Oil Oral fluid Pig's Blood Plasma Post-Mortem fluids Post-Mortem tissue Potato chips (crisps) Prawns River Water Saliva Salmon Sea Water Sediment Serum Shellfish Shrimps Sludge cake Soil Spice Stomach contents Sun Lotion Sunscreen Surface water Synthetic urine Urine Urine (horse) Vegetables Waste water Water Wheat Wheat flour Whole blood Wine (red)

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3 results

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Extraction of a Comprehensive Steroid Panel from Human Serum Using EVOLUTE® EXPRESS ABN Prior to LC/MS-MS Analysis

Part No: AN891.V.3 Issued year: 2019 File size: 4.41mb File type: pdf

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone Extrahera LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

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Extraction of a Comprehensive Steroid Panel from Human Serum Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

Part No: AN890.V.1 Issued year: 2018 File size: 1.73mb File type: pdf

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite Testosterone

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Extraction of Corticosteroids from Plasma using ISOLUTE® SLE+ Supported Liquid Extraction Plates

Part No: AN602 Issued year: 2011 File size: 0.23mb File type: pdf

This Application Note highlights the use of ISOLUTE® SLE+ supported-liquid extraction plates for the extraction of corticosteroids from human plasma, specifically Triamcinolone, Prednisolone, Hydrocortisone, Prednisone, Betamethasone, Cortisone, Dexamethasone, Flumethasone, Corticosterone, Beclomethasone, Triamcinolone, Fluocinolone, Budesonide 1, Budesonide 2 and 5-Pregnen-3β−ol-20-one.

5-pregnen-3β-ol-20-one Application Notes Beclomethasone Betamethasone Budesonide Structural Isomer 1 Budesonide Structural Isomer 2 Clinical Corticosteroids Corticosterone Cortisone Dexamethasone Flumethasone Fluocinolone Acetonide Hydrocortisone LC-MS/MS Plasma Prednisolone Prednisone Triamcinolone Triamcinolone Acetonide

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