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A Fully-automated Method for Extraction of Opiates from Urine

26 June 2019

This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using  Biotage® Extrahera™ configured for use with ISOLUTE® SLE+  supported liquid extraction columns.

The simple sample preparation procedure delivers clean extracts and analyte recoveries of greater than 74% with  RSDs lower than 10%. Using the Biotage® Extrahera™, 24  hydrolyzed samples were extracted in under 31 minutes and 24  non-hydrolyzed samples were extracted in under 36 minutes. The limits of quantitation meet or exceed the sensitivity requirements set by SAMHSA and EWDTS for workplace testing applications.


  • Dihydrocodeine
  • Codeine
  • Hydrocodone
  • Hydromorphone 
  • Morphine
  • 6-MAM
  • Oxycodone
  • Oxymorphone

Download application note below. 

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ISOLUTE® SLE+ Supported Liquid Extraction