This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The extraction protocols were ultimately transferred to an SPE automation platform and method performance versus manual processing compared.
Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems, and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. Many sample preparation approaches to the extraction of 25-hydroxy vitamin D have been employed prior to LC-MS/MS analysis. Simple protein precipitation, supported liquid extraction and complex SPE methodology are all in routine use.
This poster was first presented at MSACL 2015, Salzburg.
Update on Biotage’s COVID-19 Response Plan
Smarter Sample Preparation for Environmental Challenges
How does Biotage Act in the Corona Virus Situation?
Sample Preparation Techniques for Synthetic Benzodiazepines
Good Laboratory Practices Software Upgrade for Biotage® Extrahera™