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How to Measure Aflatoxin in Baby Milk

27 October 2014

This application note describes an SPE protocol appropriate for LC-MS/MS analysis of aflatoxin M1 found in infant formulas. The method uses ISOLUTE® Myco SPE columns and meets the requirements set in European regulations.


Aflatoxins are naturally occurring mycotoxins produced by some Aspergillus species of fungi, and they are among the most carcinogenic substances known. Aflatoxin M1 is a less harmful metabolite produced when aflatoxin is hydroxylated in the liver of cows. It passes through to the cow's milk, which is the base for infant formulas. Effective detection protocols are therefore necessary to ensure food security.

Extracted ion chromatograms in positive ion mode using ISOLUTE Myco protocol at 50 ng/L (aflatoxin M1 and aflatoxin B2 (internal standard)) from reconstituted infant formula.