This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a range of illicit and prescribed drugs.
ISOLUTE® HYDRO DME+ products provide extremely efficient removal of matrix components and hydrolysis enzyme from urine samples, using a simple pass through workflow.
Because of the enhanced sample clean up delivered by ISOLUTE® HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ). ISOLUTE® HYDRO DME+ plates and columns are ideal for urinary drugs of abuse and pain management applications because the inclusion of Biotage® HYDRO frit technology means that urine samples can be hydrolyzed in-situ in the column, eliminating the need for post hydrolysis sample transfer steps.
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