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Hydrolyze Urine Samples In-Situ - New Application Note

24 April 2019

This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a range of illicit and prescribed drugs.

ISOLUTE® HYDRO DME+ products provide extremely efficient removal of matrix components and hydrolysis enzyme from urine samples, using a simple pass through workflow.

Because of the enhanced sample clean up delivered by ISOLUTE® HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute  and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ). ISOLUTE® HYDRO DME+ plates and  columns are ideal for urinary drugs of abuse and pain management applications because the inclusion of Biotage®  HYDRO frit technology means that urine samples can be  hydrolyzed in-situ in the column, eliminating the need for post hydrolysis sample transfer steps.


  • Ecgonine methyl ester
  • Pregablin 
  • Morphine 
  • Oxymorphone 
  • Amphetamine 
  • Gabapentin 
  • Codeine 
  • 6-monoacetylmorphine 
  • MDMA 
  • Hydrocodone 
  • Mephedrone
  • Benzoylecgonine
  • Ketamine
  • 7-aminoclonazepam
  • Cocaine
  • Norbuprenorphine 
  • 7-aminoflunitrazepa 
  • Buprenorphine 
  • PCP 
  • EDDP 
  • Oxazepam 
  • Methadone
  • Zaleplon
  • Flunitrazepam and ritalinic acid

Download application note below. 

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