To facilitate the high throughput population screening of phthalate metabolites, sample preparation methods need to be simple, sensitive and robust to mitigate matrix suppression and instrument down time common to many dilute and shoot approaches to mass spectrometry. Thus, a solid phase extraction procedure was developed for these analytes. This application note details the optimization strategy for nine phthalate metabolites. Proof-of-concept for this sample preparation method was determined on a set of real patient samples (n=5). The results were in general agreement with previously reported concentration ranges for these compounds. It is anticipated that this method will have significant impact in environmental biomonitoring strategies for these analytes.
Two Alternative Approaches to the Extraction of a Suite of Regulated Veterinary Growth Promoters
Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine
New Method for Extraction of a Steroid Panel from Horse Hair
New Method for Analysis of Environmental Pesticides in Water
"I Was Surprised that Everything Worked So Well". A Researcher on Moving to Automated Sample Prep