Estrone (E1) and estradiol (E2) are female steroid hormones typically monitored in peri- and postmenopausal human serum samples to assess a number of conditions. This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum using ISOLUTE® SLE+.
An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization - two approaches often used to enhance method sensitivity. The use of ammonium hydroxide and isopropanol in the HPLC mobile phase provides a unique alternative to ammonium fluoride, allowing low levels of detection of estrogens when extracted with DCM using ISOLUTE® SLE+ for sample preparation.
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