This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Oral-Eze collection devices, prior to UPLC-MS/MS analysis.

The protocol involves sample collection as per Oral-Eze intructions, vortex mixing with ammonium hydroxide and simple extraction with ISOLUTE® SLE+ 400 μL plates or 1 mL columns. RSD’s ranged from 1.2%–9.1%.

ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.