This application note contains procedures optimized for 400 μL SLE+ columns, and 200 μL and 400 μL plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to tradition liquid liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced preparation time.
Elimination of an evaporation step between the micropulverization and supported liquid extraction clean up stages provides a streamlined procedure for hair extraction. Analytes are quantified using LC-MS/MS.
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